黑荆树愈伤组织培养及其原花色素的分析

以黑荆树叶片为外植体,在MS培养基中添加不同质量浓度的细胞分裂素6-苄氨基嘌呤(6-BA)和细胞生长素2,4-二氯苯氧乙酸(2,4-D),诱导培养出愈伤组织,采用香草醛-硫酸法和Folin-Ciocalteu法测定了不同条件处理下愈伤组织中原花色素和总多酚的含量,并用电喷雾质谱(二级质谱)法对愈伤组织中原花色素组成进行定性分析。研究结果表明:添加0.25 mg/L 6-BA和2.0 mg/L 2,4-D的培养基中,黑荆树愈伤组织诱导率及生长状况较佳,且含总多酚和原花色素分别为20.19和11.10 mg/g,明显高于其他处理,适合进行增殖培养;黑荆树愈伤组织主要由单体原花色素及低聚原花色素组成,其中以单体和二聚体居多,还有微量三聚体。

Callus Culture of Acacia mearnsii De Wilde and Analysis on Proanthocyanidin

This paper studied proanthocyanidins in callus of Acacia mearnsii De Wilde.Callus were obtained by inoculating leaf explants on MS medium , and supplemented with the combination of 6-BA and 2,4-D.The content of total phenolic compounds and proanthocyanidins in the 12 different treatments were tested by Folin-Ciocalteu assay and vanillin assay , respectively . Proanthocyanidins in callus were characterized by ESI-MS.The results showed that the leaf explants of A.mearnsii treated with 0.25 mg/L 6-BA in combination with 2.0 mg/L 2,4-D resulted in a higher rate of callus formation , better growth and maximum content of total phenolic compounds 20.19 mg/g and proanthocyanidins 11.10 mg/g, which is suitable for proliferation culture . We detected monomer , dimer and trace trimer in callus of A.mearnsii, too.