Norway spruce die-back: isolation,biological activity,measurement of concentration of p-hydroxy acetophenone and its O-glucoside (Picein) by gas chromatography

This short report as a part of a broad work (Eur. J. For. Path. 15, 1985a) describes the isolation, the biological activity and the measurement of concentration of p-hydroxy acetophenone and its O-gluco-side (picein) by gas chromatography. p-hydroxy acetophenone was found to be phytotoxic to the 2-year-old saplings at a conc. of 500 ppm. The gas chromatographic measurement of p-hydroxy aceto-phenone (p-HAP) and its O-glucoside (after enzymic hydrolysis) in the current year's shoot parts showed the ratio p-HAP: p-HAP-O-glucoside always larger than 1 in the affected trees and less than 1 in the healthy trees.