神经内分泌因子、代谢产物对体外培养新生犊牛肝细胞胰高血糖素受体mRNA丰度的影响

为阐明神经内分泌因子胰岛素（In）、胰高血糖素（GLN）、瘦素（LEP）和代谢产物非酯化脂肪酸（NEFA）、β-羟丁酸（BHBA）、葡萄糖（GLU）在奶牛肝糖代谢、脂代谢中的调控作用，应用荧光定量PCR（fluorescent quantitative PCR）法观察了神经内分泌因子、代谢产物对体外培养新生犊牛肝细胞胰高血糖素受体（glucagon receptor，GLNR）mRNA丰度的影响。结果显示：随着培养液中In、NEFA、BHBA和GLU的升高，GI。NRmRNA表达逐渐增加（P〈0．01）；随着培养液中GLN浓度的升高，GLNRmRNA表达逐渐降低（P〈0．01）；而随着培养液中瘦素浓度的升高，GLNRmRNA的表达呈先升高后降低的趋势（P〈0．01）。表明：神经内分泌因子In、GLN、LEP和代谢产物NEFA、BHBA、GLU直接调控新生犊牛肝细胞GLNRIT／RNA的表达。

Effects of Neuroendocrine Factors and Metabolites on Expression of GLNR mRNA by Neonatal Newborn Calf Hepatocytes Cultured in Vitro

In order to investigate the regulation roles of neuroendocrine factors and metabolites in hepatocyte glycometabolism and lipometabolism of dairy cows,this study observed the effects of bovine Insulin and glucagon on expression of glucagon receptor(GLNR)mRNA of cultured neonatal calf hepatocytes by real-time fluorescent quantitative PCR.The expression of GLNR mRNA increased(P<0.01)with increasing concentrations of In NEFA BHBA and GLU in the culture media;and when glucagon in the culture media increased gradually,the expression of GLNR mRNA decreased(P<0.01).Along with increase of leptin in the medium,the expression of GLNR increased at first and then decreased(P<0.01).These results indicate that neuroendocrine factors and metabolites in the media can directly regulate the expressions of GLNR mRNA in the neonatal calf hepatocytes in vitro.