| 芦荟原生质体融合的研究 |
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| 引用本文： | 王红霞,王文锋. 芦荟原生质体融合的研究[J]. 山东农业科学, 2010, 0(10): 27-29 |
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| 作者姓名： | 王红霞 王文锋 |
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| 作者单位： | 新乡医学院生命科学技术系,河南,新乡,453003 |
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| 基金项目： | 2009年河南省教育厅立项项目(编号:2009A180014) |
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| 摘 要： | 以芦荟叶片为试材,采用酶解方法进行原生质体分离,并在此基础上对斑纹芦荟和不夜城芦荟进行原生质体融合。结果表明,以芦荟的叶肉细胞为试材,可成功分离出原生质体,而且活力较高,达90%以上。聚乙二醇（PEG）融合的结果可获得部分融合体。芦荟原生质体的成功分离主要取决于材料的来源、酶液的组成,另外还受酶解液的渗透压、温度和pH值等影响。融合的效率主要受融合剂的浓度、原生质体的生理状态及处理的时间等影响。
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| 关 键 词： | 芦荟 原生质体 分离 融合 |
Study on Protoplast Fusion of Aloe vera L. |
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| WANG Hong-xia,WANG Wen-feng. Study on Protoplast Fusion of Aloe vera L.[J]. Shandong Agricultural Sciences, 2010, 0(10): 27-29 |
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| Authors: | WANG Hong-xia WANG Wen-feng |
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| Affiliation: | WANG Hong-xia,WANG Wen-feng(Life Science , Technique Department of Xinxiang Medical College,Xinxiang 453003,China) |
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| Abstract: | The protoplast of Aloe vera L.was isolated from its leaves by enzymolysis method.Then the protoplast fusion of Aloe nobilis and Aloe vera L.var.chenensis was induced by PEG.As a result,the protoplast was successfully gotten from the mesophyll cells of Aloe vera L.with the higher activity over 90%.Some fusants were obtained by PEG induction successfully.Protoplast isolation of Aloe vera L.was mainly related with the plant material and the component of enzyme solution,and also affected by osmotic pressure,tem... |
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| Keywords: | Aloe vera L. Protoplast Isolation Fusion |
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