家鸡冠型性状的微卫星标记连锁分析

 【目的】构建具有冠型性状记录的鸡F2资源群的2号染色体连锁图谱,并结合表型分析对调控鸡冠型性状的基因进行初步定位。【方法】通过测交选择豆冠冠型基因型纯合的吐鲁番斗公鸡2只和玫瑰冠冠型基因型纯合的玫瑰冠母鸡6只为亲本,采用F2代试验设计建立F2代资源群436只,参考EL（East Lansing）家系的遗传连锁图谱,在鸡2号染色体上筛选14对微卫星标记,运用MapMaker/EXP3.0软件初步构建此家系2号染色体遗传连锁图谱,同时运用LOD记分法进行鸡的冠型基因连锁分析。【结果】经χ2检验,F2资源群冠型分化比例符合经典孟德尔遗传学的独立分离规律。在连锁分析中除MCW0157外,其余13个微卫星标记均能连锁,所构建的鸡2号染色体连锁图谱与EL家系的遗传连锁图谱相似。【结论】鸡2号染色体上MCW0082位点与玫瑰冠冠型基因可能存在连锁。

Microsatellite Markers' Linkage Analysis of the Comb Traits of Chicken

【Objective】 To establish a linkage map of the chromosome 2 in the chicken-F2 resource group with cockscomb trait record, and to determine a preliminary mapping for the gene of controlling comb trait combined with the phenotypic analysis.【Method】 Through test cross, 2 Tulufan fighting cocks with the homozygous pea-comb genotype and 6 rose comb hens with the homozygous rose-comb genotype were chosen as the parents. Four hundred and thirty six chickens of the F2 generation group were built by the F2 generation experimental design, and the genetic linkage map of EL (East Lansing) family was also referred. Meanwhile, the 14 microsatellite markers on the chromosome 2 of chicken were selected, and the family genetic linkage map of chromosome 2 was built preliminarily by using MapMaker/EXP3.0 software. In addition, the linkage analysis of cocks comb gene was completed through the LOD scoring method. 【Result】 By the χ2 test, the proportion of differentiation of the F2 resource group was accorded with the law of independent segregation of classic Mendelian inheritance. Except for the MCW0157, the other 13 microsatellite markers all could have linkage, and the genetic linkage map of chromosome 2 in this family was similar with the EL family’s. 【Conclusion】 The site MCW0082 of chicken chromosome 2 may have linkage with the rose comb gene.