基于高通量测序技术分析使它隆对玉米根系内生菌多样性的影响

利用Illumina Miseq高通量测序技术,对玉米根系内生菌16S rRNA的V4~V5可变区序列进行测定,分析不同时期有杂草生长（长草组）与使用使它隆除草对玉米根系内生菌群落组成的影响。从5个玉米根系样品共得到166 647条有效序列、145 298条优质序列和4 464个细菌OTUs。Alpha多样性分析结果表明,玉米根系内生菌的群落多样性随玉米植株的生长而增加;使用使它隆除草后,群落的丰富度和均匀度在花期下降,在成熟期下降明显。对群落组成分析发现,使用使它隆除草后,与长草组相比,花期和成熟期的内生菌在门及属水平上的分布均发生较大变化。内生菌的基因功能分析表明,使用使它隆除草后,花期根系内生菌的异型生物质的降解代谢通路丰度高于未喷施使它隆的长草组样品;成熟期糖代谢通路丰度高于长草组。结果表明,喷施使它隆能降低玉米根系内生菌的多样性,并使内生菌的组成比例发生变化。花期根系内生假单胞菌属等比例的增加与异型生物质的降解代谢有关;成熟期肠杆菌属、芽孢杆菌属等比例的增加与糖代谢功能的提高有关。

Effects of Starane on the Community Diversity of Maize Root Endophytes Analyzed Using High-Throughput Sequencing Technology

The Illumina Miseq high-throughput sequencing technology was used to determine the V4-V5 variable region sequence of 16S rRNA and the effect of growing weed or weeding control with starane on the composition of the community structure of maize root endophytes in different periods. The research received a total of 166 647 effective sequences, 145 298 high-quality sequences, 4 464 OTUs from 5 maize root samples. The results of the analysis of Alpha diversity showed that the community diversity of maize root endophytes increased with the growth of maize plants. By weeding control with starane, the richness and evenness of the root community were decreased slightly at florescence stage, and obviously at maturation stage. Community composition analysis showed that by weeding control with starane, the distribution of bacteria in phylum and genus level changed greatly at florescence and maturation stages compared with the grass groups. The analysis of the gene function of endophytes showed that by weeding control with starane, the abundance of xenobiotics biodegradation metabolism was higher than the group of growing weed at florescence stage, and the abundance of carbohydrate metabolism increased at maturation stage. In brief, the result indicated that the spraying starane reduced the diversity of bacteria in maize roots, and the proportion of endophytic bacteria changed. The increase in the proportion of Pseudomonas was relevant with the xenobiotics biodegradation metabolism at florescence stage, and the increase in proportion of Enterobacter and Bacillus was relevant with the increase of carbohydrate metabolism.