| 重组人表皮生长因子基因遗传转化烟草研究 |
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| 引用本文： | 刘洋,徐成武,赵德刚. 重组人表皮生长因子基因遗传转化烟草研究[J]. 分子植物育种, 2008, 6(3): 465-470 |
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| 作者姓名： | 刘洋 徐成武 赵德刚 |
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| 作者单位： | 贵州大学生命科学学院贵州省农业生物工程重点实验室,贵阳,550025;贵州大学教育部绿色农药与农业生物工程重点实验室,贵阳,550025;贵州大学生命科学学院贵州省农业生物工程重点实验室,贵阳,550025 |
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| 基金项目： | 贵州省优秀青年科技人才培养专项基金 , 教育部春晖计划项目 |
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| 摘 要： | 将携带内质网滞留信号肽SEKDEL编码序列的重组人表皮生长因子基因（hEGF）克隆到植物表达载体中构建植物表达载体pSH-hEGFS,通过冻融法将pSH-hEGFS转化到根癌农杆菌LBA4404中,以烟草无菌苗叶盘为外植体,通过农杆菌介导法进行遗传转化,经抗生素抗性筛选获得抗性植株,通过GUS组织化学染色分析、PCR检测以及RT-PCR分析,证明重组人表皮生长因子基因已整合到烟草基因组中并已表达,间接法ELISA检测结果表明转基因烟草中重组人表皮生长因子表达量最高达叶片总可溶蛋白的0.003%。
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| 关 键 词： | 人表皮生长因子 遗传转化 烟草 内质网滞留信号肽 |
Genetic Transformation of Tobacco with Human Epidermal Growth Factor Gene |
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| Liu Yang,Xu Chengwu,Zhao Degang. Genetic Transformation of Tobacco with Human Epidermal Growth Factor Gene[J]. Molecular Plant Breeding, 2008, 6(3): 465-470 |
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| Authors: | Liu Yang Xu Chengwu Zhao Degang |
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| Affiliation: | Liu Yang 1,2 Xu Chengwu 1 Zhao Degang 1,21 Guizhou Key Laboratory of Agro-Bioengineering,College of Life Sciences,Guizhou University,Guiyang 550025,2 The Key Laboratory of Green Pesticide , Agricultural Bioengineering,Ministry of Education,Guiyang,550025 |
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| Abstract: | The expression vector pSH-hEGFS carrying a human epidermal growth factor gene and SEKDEL as endoplasmic reticulum orientation sequence was constructed and introduced into Agrobacterium tumefaciens strain LBA4404 by Freeze-Thaw method. The tobacco leaf disc was transformed by LBA4404. Regenerated plantlets were obtained via co-culture, shoot inducing and root inducing. Transgenic plants were conformed by the GUS histochemistry analysis, PCR analysis and RT-PCR analysis. The results indicated that the hEGF ge... |
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| Keywords: | hEGF Genetic transformation Tobacco SEKDEL |
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