| 实时荧光定量PCR筛选牛源整联蛋白αv基因的siRNA片段 |
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| 引用本文： | 张国峰,独军政,常惠芸,薛霜,骆继怀.实时荧光定量PCR筛选牛源整联蛋白αv基因的siRNA片段[J].动物医学进展,2011,32(3):56-60. |
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| 作者姓名： | 张国峰 独军政 常惠芸 薛霜 骆继怀 |
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| 作者单位： | 中国农业科学院兰州兽医研究所家畜疫病病原生物学国家重点实验室,农业部畜禽病毒学重点开放实验室,国家口蹄疫参考实验室,甘肃兰州,730046 |
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| 摘 要： | 设计并合成三条针对牛口蹄疫病毒(FMDV)整联蛋白受体αv亚基基因的特异性干扰小RNA(SiRNA)片段,将其转染至本身表达整联蛋白受体αv亚基基因的MDBK细胞.分别在转染后36 h和48 h收集细胞,提取细胞总RNA并反转录为cDNA.应用SYBR GreenI实时荧光定量RT-PCR的方法检测3个siRNA片段的...
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| 关 键 词： | 实时荧光定量PCR 整联蛋白 RNA干扰 SYBR Green Ⅰ |
Selection of siRNA Fragment of Cattle-borne Integrin-αv Gene by Real-time Fluorescence Quantitative PCR |
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| ZHANG Guo-feng,DU Jun-zheng,CHANG Hui-yun,XUE Shuang,LUO Ji-huai.Selection of siRNA Fragment of Cattle-borne Integrin-αv Gene by Real-time Fluorescence Quantitative PCR[J].Progress In Veterinary Medicine,2011,32(3):56-60. |
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| Authors: | ZHANG Guo-feng DU Jun-zheng CHANG Hui-yun XUE Shuang LUO Ji-huai |
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| Institution: | ZHANG Guo-feng,DU Jun-zheng,CHANG Hui-yun,XUE Shuang,LUO Ji-huai(State Key Laboratory of Veterinary Etiological Biology,Key Laboratory of Animal Virology of Ministry of Agriculture,National FMD Reference Laboratory,Lanzhou Veterinary Research Institute,Chinese Academy of Agricultural Sciences,Lanzhou,Gansu,730046,China) |
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| Abstract: | |
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| Keywords: | real-time PCR integrin RNA interference SYBR greenⅠ |
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