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鸡包涵体肝炎的病原检测及病理学观察
引用本文:李海英,尹燕博,王建琳,郭妍妍,王晓红,王守春,张毅,王光,李吉达.鸡包涵体肝炎的病原检测及病理学观察[J].动物医学进展,2010,31(7):55-60.
作者姓名:李海英  尹燕博  王建琳  郭妍妍  王晓红  王守春  张毅  王光  李吉达
作者单位:1. 青岛农业大学动物科技学院,山东青岛,266109
2. 青岛澳兰百特生物工程有限公司,山东青岛,266101
3. 中国农业大学动物医学院,北京,100193
摘    要:2007年和2008年山东某鸡场相继暴发某疫病,为了确定病原和了解患病鸡死亡原因,分别对患病鸡进行了PCR检测、动物回归试验,并同时进行了病理学观察。结果表明,光镜下肝脏严重变性、坏死,肝细胞核内形成嗜酸性或嗜碱性核内包涵体的特征性病变。电镜下也观察到大量呈典型的晶格状排列的禽腺病毒粒子。说明鸡包涵体肝炎过程中,肝脏和肾脏的严重损伤所引起的代谢紊乱,可能是患鸡死亡的直接或间接原因。

关 键 词:  包涵体肝炎  聚合酶链反应  六邻体蛋白基因  病理学观察

Pathological Observation of Chicken Inclusion Body Hepatitis
LI Hai-ying,YIN Yan-bo,WANG Jian-lin,GUO Yan-yan,WANG Xiao-hong,WANG Shou-chun,ZHANG Yi,WANG Guang,LI Ji-da.Pathological Observation of Chicken Inclusion Body Hepatitis[J].Progress In Veterinary Medicine,2010,31(7):55-60.
Authors:LI Hai-ying  YIN Yan-bo  WANG Jian-lin  GUO Yan-yan  WANG Xiao-hong  WANG Shou-chun  ZHANG Yi  WANG Guang  LI Ji-da
Institution:LI Hai-ying1,YIN Yan-bo1,WANG Jian-lin1,GUO Yan-yan2,WANG Xiao-hong1,WANG Shou-chun1,ZHANG Yi3,WANG Guang1,LI Ji-da1 (1.College of Animal Science and Veterinary Medicine,Qingdao Agricultural University,Qingdao,Shandong,266109,China,2.Oland-better Bionengineering Company,266101,3.College of Veterinary Medicine,China Agriculture University,Beijing,100193,China)
Abstract:The viruses of certain disease was isolated from a farm of Shandong province where the disease had been outbroken in the year of 2007and 2008.In order to determine the pathogen and know the causes of death in infected chickens,the PCR,animal regression testing and pathological research were carried out.The results showed that infected chickens not only had charateristic pathologic lesions such as serious degeneration,necrosis,formation of eosinophilic inclusion or basophilia inclusion bodies in hepatic cell...
Keywords:chicken  inclusion body hepatitis  PCR  hexon protein gene  pathological observation  
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