应用重组自交系群体检测控制水稻糙米粗蛋白和粗脂肪含量的QTL

用协青早B/密阳46重组自交系群体及其分子连锁图谱，及Windows QTL Cartographer 2.0的复合区间作图法和多区间作图法，对水稻糙米蛋白质含量和粗脂肪含量进行QTL分析。检测到控制蛋白质含量的QTLs 5个（qPc-3、qPc-4、qPc-5、qPc-6、qPc-10），单个QTL对群体表型变异的贡献率

QTL Mapping of Brown Rice Protein Content and Lipid Content in A Recombinant Inbred Population of Rice

Protein content and lipid content are two important traits in evaluation of nutrient quality of rice.Rice is a major protein source in most rice-eating areas and protein can influence the physicochemical properties of cooked rice.Lipid also affects the cooking and eating quality of rice.The objective of this study was to identify QTLs conditioning brown rice protein content and lipid content using a recombinant inbred line(RIL)population derived from a cross between Xieqingzao B and Milyang 46 and its genetic linkage map by the composite interval mapping(CIM) and multiple interval mapping(MIM) of Windows QTL Cartographer 2.0.Five QTLs(qPc-3,qPc-4,qPc-5,qPc-6,qPc-10) and four QTLs(qLc-3,qLc-5,qLc-6,qLc-8) showing significant additive effects for protein content and lipid content were detected,respectively.The five QTLs for protein content collectively explained 42.8% of the phenotypic variance,with the variance explained by a single QTL ranged from 4.7% to 19.4%.The four QTLs for lipid content collectively explained 34.6% of the phenotypic variance,with the variance explained by a single QTL ranged from 5.5% to 12.0%.QTLs located in the vicinity of Wx gene on chromosome 6 displayed a major effect for protein content and a considerable effect for lipid content.Utilization of the QTL mapping on marker assisted selection for the improvement of grain quality in rice was also discussed.