| 大豆蛋白质相关基因SNP的开发和遗传定位 |
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| 引用本文： | 蔡春梅,柳伟先,李锡河.大豆蛋白质相关基因SNP的开发和遗传定位[J].延边大学农学学报,2009,31(2):77-83. |
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| 作者姓名： | 蔡春梅 柳伟先 李锡河 |
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| 作者单位： | 1. 青岛农业大学生命科学学院,山东,青岛,266109
2. 韩国首尔大学农业与生命科学学院,韩国,首尔,151921 |
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| 摘 要： | 在大豆蛋白质相关基因中利用直接测序法在2个代表性品种Pureukong与Jinpumkong2之间发掘SNP标记，并利用这些标记将蛋白质相关基因定位在遗传图谱上．为了开发SNP分子标记，选择56个蛋白质相关基因并设计相应的引物．结果显示，49个引物获得较好的测序结果．其中，7个引物在2个亲本之间显示多态性，共检测出11个SNP分子标记．2个亲本间SNP的检测频率为1SNP／2400bp．利用杂交获得的重组自交系，大豆蛋白质相关基因被定位到已经构建的以SSR分子标记为主的遗传图谱上．
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| 关 键 词： | 大豆 SNP 蛋白质 |
SNP discovery and linkage analysis of protein related genes in Soybean |
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| CAI Chun-mei,LIU Wei-xian,LI Xi-he.SNP discovery and linkage analysis of protein related genes in Soybean[J].Journal of Agricultural Science Yanbian University,2009,31(2):77-83. |
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| Authors: | CAI Chun-mei LIU Wei-xian LI Xi-he |
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| Institution: | CAI Chun-mei, LIU Wei-xian, LI Xi-he (1. Life Sciences College of Qingdao Agricultural University, Qingdao Shandong , 266109, China ; 2. Agricultural and Life Sciences College of Seoul National University, Seoul 151921,Korea) |
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| Abstract: | Found SNPs between Purenkong and Jinpumkong 2 using direct sequencing method and to map these genes to soybean linkage map. To discover SNPs, a total of 76 protein related genes were selected and primers were designed. Among 49 loci showing high-quality sequence, 11 SNPs were identified in 7 loci between the parental genotypes. Average SNP frequency between the parental genotypes was one SNP per 2 400 bp. With SNP genotyping in 90 RILs, these genes were mapped in exiting linkage map. |
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| Keywords: | SNP |
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