| 利用转基因技术获得抗病陆地棉的初步研究 |
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| 引用本文： | 王冬梅,李建平,张艳红,周小云,黄乐平.利用转基因技术获得抗病陆地棉的初步研究[J].新疆农业科学,2007,44(Z3):108-110. |
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| 作者姓名： | 王冬梅 李建平 张艳红 周小云 黄乐平 |
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| 作者单位： | 新疆农科院核生所,乌鲁木齐,830091 |
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| 基金项目： | 新疆农作物细胞工程重点实验室课题 |
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| 摘 要： | 由于棉花的枯、黄萎病日益严重,选育抗病品种成为育种的首要任务。为提高棉花的抗病性,利用花粉管通道法将从拟南芥中分离克隆的抗病基因snc1转入中棉所35号中,通过除草剂田间筛选和目的基因的PCR检测,获得转snc1基因的棉花,同时确定除草剂的筛选浓度为0.025%。也为棉花的抗病机理研究做有益探索。
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| 关 键 词： | 抗病基因snc1 花粉管通道法 陆地棉 |
| 文章编号： | 1001-4330(2007)S3-0108-03 |
| 修稿时间： | 2007年10月24 |
Preliminary Study on Obtaining Disease - resistant Upland Cotton by Transgenic Technology |
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| WANG Dong-mei,LI Jian-ping,ZHANG Yan-hong,ZHOU Xiao-yun,HUANG Le-ping.Preliminary Study on Obtaining Disease - resistant Upland Cotton by Transgenic Technology[J].Xinjiang Agricultural Sciences,2007,44(Z3):108-110. |
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| Authors: | WANG Dong-mei LI Jian-ping ZHANG Yan-hong ZHOU Xiao-yun HUANG Le-ping |
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| Abstract: | When cotton wilt is becoming increasingly serious it is very important to breed disease-resistance cotton.In order to crease the resistance of cotton,by means of pollen-tube pathway,snc1(supressor of npr1-1,constitution 1) cloned from Arabidopsis was transferred into "Zhongmiansuo 35".By selecting of bastar and PCR,transgenic cottons were obtained,and selecting the concentration of bastar was 0.025%.It is useful exploration to study mechanism of disease-resistance cotton. |
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| Keywords: | disease-resistant gene pollen-tube pathway upland cotton |
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