Deeply inverted electron-hole recombination in a luminescent antibody-stilbene complex |
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Authors: | Debler Erik W Kaufmann Gunnar F Meijler Michael M Heine Andreas Mee Jenny M Pljevaljcic Goran Di Bilio Angel J Schultz Peter G Millar David P Janda Kim D Wilson Ian A Gray Harry B Lerner Richard A |
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Affiliation: | Department of Molecular Biology, Scripps Research Institute, La Jolla, CA 92037, USA. |
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Abstract: | The blue-emissive antibody EP2-19G2 that has been elicited against trans-stilbene has unprecedented ability to produce bright luminescence and has been used as a biosensor in various applications. We show that the prolonged luminescence is not stilbene fluorescence. Instead, the emissive species is a charge-transfer excited complex of an anionic stilbene and a cationic, parallel pi-stacked tryptophan. Upon charge recombination, this complex generates exceptionally bright blue light. Complex formation is enabled by a deeply penetrating ligand-binding pocket, which in turn results from a noncanonical interface between the two variable domains of the antibody. |
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