不同日龄断奶应激对仔猪血液生化和免疫指标的影响

[目的]研究不同断奶日龄仔猪的血液生化和免疫指标的变化情况。[方法]选用14日龄和28日龄断奶杂交仔猪,于断奶次日和断奶后每隔7d采血直至42日龄,测定仔猪血液乳酸脱氢酶LDH、总蛋白TP、白蛋白ALB、球蛋白GLOB和免疫指标IgG、IgM、IgA的含量。[结果]不同日龄断奶均使仔猪在断奶后7d内遭受严重的应激反应,LDH活力明显上升（P﹤0.05或P﹤0.01）,IgG、IgM、IgA和TP、ALB、GLOB水平迅速下降（P﹤0.05或P﹤0.01）。14日龄和28日龄断奶仔猪分别在断奶后21和14d与对照组相比,LDH活性差异不显著（P﹥0.05）。试验组仔猪42日龄时（除28日龄断奶仔猪ALB外）,IgG、IgM、IgA、TP、ALB和GLOB水平与对照组相比仍然差异显著（P﹤0.01或P﹤0.05）,但28日龄断奶仔猪与14日龄断奶仔猪相比免疫球蛋白水平和血清蛋白水平下降较平缓。[结论]不同日龄断奶仔猪断奶后均遭受断奶应激,相对14日龄断奶仔猪而言,28日龄断奶仔猪遭受的应激程度较小;血清LDH活性可作为判断仔猪应激程度的参考指标。

Effects of Weaning Stress at Different Ages on the Serum Biochemical and Immune Indices of Piglets

[Objective] The research aimed to study on the changes of serum biochemical and immune indices in piglets with different weaning ages. [Method] 14-day-old and 28-day-old weaned hybrid piglets were selected. The blood samples were collected on the next day of weaning and every other week until 42 days old to determine the serum LDH, TP, ALB, GLOB, IgG, IgM and IgA. [Result] The piglets of different weaning age were suffered severely weaning stress after weaning 21 d (test group 1) and 14 d (test group 2), compared with control compared with that IgG, IgM, IgA, TP, ALB and GLOB (except ALB level in 28 d immunoglobulin levels and serum protein levels were decreased moderately in 28 d weaned piglets, compared with 14-d weaned piglets. [Conclusion] The piglets at different weaning age were suffered weaning stress after weaning, 28 d weaned piglets have less weaning stress, compared with 14 d weaned piglets, and the index of LDH can be used as a reference to determine the weaning stress degree in pig production.