甜菜夜蛾核型多角体病毒（SeNPV）对非宿主细胞的病理影响

甜菜夜蛾（Spodoptera exigua）核型多角体病毒（SeNPV）克隆株G1、G3、G4及SeNPV1感染SpIm、BmN4和Ld652y细胞报，均能引起细胞凋亡现象。SpIm细胞感染96h后，大部分细胞死亡，形成葡萄状小泡，但能在个别细胞中形成病毒多角体；BmNr4细胞感染48h后，细胞内颗粒状物质增多，72h后全部死亡，脱壁上浮；Ld652y细胞被G3及SeNPV1感染 72h后解体成颗粒状，而G1、G4只能令部分细胞死亡。Westreren Blot及SlotBlot分析表明：SeNPV克隆株能在SpIm及BmN4细胞中复制病毒DNA，且能在SpIm细胞中形成多角体蛋白，但不能在Ld652y细胞中复制DNA。

The Influrence on the Pathologic Characterics of Non Host Cell Line Byinfecting with the Nuclear Polyhedrosis Virus of Beet Armyworm,Spodoptera exigua

The phenomenon of programmed cell death was observed in the non host cell lines Ld652y(Lymantria dispar), BmN4(Bombyx mori)andSpIm( Sphilosoma imparilis)by infecting with the clones of G1,G3,G4 and SeNPV of nuclear polyhedrons virus from army worm(Spodoptera xigua,Se).After infected with SeNPV,the Ld652y cell died and some granular were found in the cell;the BmN4 cell left the wall and float in the medium;the SpIm cell developed some grape-shape vacuoles and the polyhedrin bodies were found in few cells.The result of Westreren Blot and Slot Blotshowed that the SeNPV DNA can replicate in both SpIm and BmN4 cell, but the polyhedrin protein developed in SpIm cell only.The pathologic characterizes of SeNPV infection were different between Ld652y and SpImcell lines.