苏丹草品种纯度鉴定技术的研究——过氧化物酶同功酶标记

对我国审定登记的5个苏丹草品种，应用幼苗叶片过氧化物酶同功酶电泳技术进行品种鉴定，结果表明，5个品种过氧化物酶同功酶表现出明显的谱带差异：首先根据相对迁移率（Rf）为0.834位置酶带的形状将5个品种划分为3组。其中，新苏2号和盐池两品种呈连续的条形，宁农和乌拉特1号下方有1三角形谱带，而奇台苏丹草在该谱带左下方有1圆点，其次，新苏2号和盐池两品种在Rf为0.455和0.493位置存在酶带，但新苏2号酶带颜色明显深于盐池，另外，宁农和乌拉特1号棹比，Rf为0.834位置下方的三角形谱带乌拉特1号居中而宁农偏右，可据此进一步区分该2品种。本研究认为过氧化物酶同功酶是苏丹草品种鉴定的快速有效技术。

Study on technology of variety identification in sudangrass--POD isozyme marking

Suitability of seedling POD isozyme PAGE was studied for identifiing 5 varieties of sudangrass (Sorghum sudanense) registered in China. Result showed that patterns of POD isozyme PAGE were markedly different among the varieties. Three groups were, firstly classified according to shape of band at of Rf 0.834. Among them, Xinsu No.2 and Yanchi belong to one group being with continuous stripe band; Qitai has a distinguish band of dot shape at left down side; and the other two varieties have a distinguish triangle band below Rf 0.834. Secondly, there are two bands are in common at Rf 0.455 and 0.493 between the varieties of Xinsu No.2 and Yanchi, however, the band of Xinsu No.2 is more darker than Yanchi. In addition, between the varieties of Ningnong and Wulate No.1, the triangle band at Rf 0.834 for Ningnong is located at right side, while that for Wulate No.1 is in the middle. This study suggested that the seedling POD isozyme PAGE could be as an efficient technique for variety identification in sudangrass.