Development of a novel diagnostic test for detection of bovine viral diarrhea persistently infected animals using hair |
| |
| Authors: | Kuldeep Singh Myrna M. Miller Laura J. Kohrt Gail Scherba Edgar F. Garrett Richard L. Fredrickson |
| |
| Affiliation: | 1Veterinary Diagnostic Laboratory, College of Veterinary Medicine, University of Illinois, Urbana, IL 61802, USA.;2Veterinary Teaching Hospital, College of Veterinary Medicine, University of Illinois, Urbana, IL 61802, USA.;3Department of Veterinary Science, Wyoming State Veterinary Laboratory, Laramie, WY 82070, USA. |
| |
| Abstract: | The purpose of this study was to determine whether manually plucked hairs might serve as an alternative sample for a quantitative real time polymerase chain reaction (qRT-PCR) testing. Twenty three, 1~3 week old, non-bovine viral diarrhea virus (BVDV) vaccinated calves, found to be positive for BVDV by immunohistochemical staining, were selected and hairs were manually plucked from the ear. qRT-PCR was performed on samples consisting of more than 30 hairs (30~100) and whole blood. All 23 animals were positive for the virus by qRT-PCR performed on the whole blood and when samples of more than 30 hairs were assayed. Additionally, qRT-PCR was performed on groups of 10 and 20 hairs harvested from 7 out of 23 immunohistochemical staining-positive calves. When groups of 20 and 10 hairs were tested, 6 and 4 animals, respectively, were positive for the virus. |
| |
| Keywords: | bovine viral diarrhea virus diagnostic tests hair persistent infection RT-PCR |
|
|
