与茄子果皮颜色相关联的AFLP及SCAR标记

 【目的】寻找与茄子皮色或果皮花青素合成相关基因连锁的分子标记,检验混合品系法（bulked lines analysis）在筛选分子标记中的应用。【方法】采用AFLP技术,以具有不同果皮颜色的茄子及近缘种为材料,采用单株检测和混合品系法分别筛选与茄子果皮颜色相关基因连锁的分子标记。【结果】对58份高代自交系材料进行单株检测（亲缘关系分析）,筛选到了1个与茄子紫红、紫黑果色相关的共显性标记。测序结果表明,片段长度分别为107和108 bp,为发生插入/缺失突变的同源序列;将其转化为SCAR标记,对136份茄子材料进行验证,在其中111份紫红、紫黑皮色材料中,相关符合率为90%,在白色及绿色材料中表现为不规律分布。最后以混合品系法,在两池内进一步筛选到了6个多态性标记,对6个标记进行单株验证,确定了它们与紫红、紫黑果色相关。【结论】本试验所获得的SCAR标记、AFLP标记可以用于茄子果色分子标记辅助育种。

AFLP and SCAR Markers Associated with Peel Color in Eggplant

Objective] The study identified the molecular marker linked to the gene which controlled fruit color (fc) or fruit anthocyanin biosynthesis in eggplant, and tested the application of bulked line analysis in identifying molecular markers. Method] With the method of assessing individuals and bulked line analysis, the AFLP technique of DNA analysis was employed to screen the markers linked to gene of interest among 136 accessions including 130 Solanum melongena and 6 relatives. Result] One codominant AFLP marker was identified to be related to the peel color of black purple and purplish red after screening 58 advanced inbred lines individually. Sequencing of the polymorphic fragment indicated that their lengths were 107 bp and 108 bp, respectively, and they were orthologous sequences with an insertion/deletion of only one nucleiotide. The AFLP marker was further converted into SCAR marker, The analysis on another set of 78 accessions, combining with previous data, indicated that the SCAR marker associated significantly with the black purple and purplish color of fruit peel, but with no correlation with other colors. Beside the above marker, another 6 markers showing polymorphic between black purple fruit pool and purplish red ones were also detected using bulked line analysis, and all of them were demonstrated to be highly correlated to the fruit purple color of eggplant as the SCAR marker when tested on the individual lines. Conclusion] The generated SCAR markers and AFLP markers can be used in molecular marker-assisted breeding for fruit peel color in eggplant.