| 短小芽孢杆菌碱性蛋白酶的提纯与性质研究 |
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| 引用本文： | 褚忠志,于新.短小芽孢杆菌碱性蛋白酶的提纯与性质研究[J].甘肃农业大学学报,2008,43(6). |
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| 作者姓名： | 褚忠志 于新 |
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| 作者单位： | 1. 甘肃农业大学食品科学与工程学院,甘肃,兰州,730070
2. 广东仲恺农业技术学院,广东,广州,51000 |
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| 基金项目： | 国家自然科学基金，广东省自然科学基金，广东省科技厅科技计划 |
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| 摘 要： | 将短小芽孢杆菌(Bacillus pumilus)Zkud202-4液体的发酵液离心去菌体,用硫酸铵盐析得粗酶,透析除盐后进行Sephadex G-75柱层析得到电泳纯碱性蛋白酶,用该法提纯的碱性蛋白酶比活力从粗酶液的155.5 U/mg提高到了954 U/mg,回收率为27.6%.该酶水解酪蛋白的最适反应温度为50℃,最适作用pH为10,且该酶具有较高的热稳定性和耐碱性.经SDS-PAGE测定,其分子质量约为2.5 ku.
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| 关 键 词： | 短小芽孢杆菌 碱性蛋白酶 提取纯化 性质 |
Purification and partial properties of alkaline protease Zkud202-4 from Bacillus pumilus |
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| CHU Zhong-zhi,YU Xin.Purification and partial properties of alkaline protease Zkud202-4 from Bacillus pumilus[J].Journal of Gansu Agricultural University,2008,43(6). |
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| Authors: | CHU Zhong-zhi YU Xin |
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| Abstract: | The fermented liquit medium by Bacillus pumilus strain Zkud202-4 was centrifuged,and the supernatants were purified through the process involving ammonium sulfate precipitation,Sephadex G-75 filtration.The specific activity of enzyme was increased from 155.5 U/mg to 954 U/mg with the recovery of 27.6 %.The optimal temperature and pH for casein hydrolysis were 50 ℃ and 10 respectively.The purified proteinase was demonstrated to be heat stable and suitable in alkaline.The alkaline protease of electrophoretic homogeneity by SDS-PAGE showed the product was a protein with a molecular weight of 2.5 ku. |
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| Keywords: | Bacillus pumilus alkaline protease purification property |
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