基于14C同化物运转和分配对黄柏剥皮的再生机理

以14C为示踪剂,用14CO2+CO2法对2年生黄柏进行半株标记,在主干上环状剥去韧皮部,以塑料薄膜包裹,燃烧法制样、液体闪烁测量法测14C活度。结果表明,未剥皮黄柏韧皮部的14C同化物运输速率为52.0cm/h,3h左右同化物从树冠运输到根系,树冠不同部位的功能叶14C同化物分配为:中部功能叶>上部功能叶>下部功能叶;剥皮后,同化物分配规律改变为:上部功能叶>中部功能叶>下部功能叶;剥皮第15天在新生韧皮部、剥口下方的树皮及根系中检测到14C同化物,表明新生韧皮部的生理功能得到恢复。

BARKED REGENERATION MECHANISM OF Phellodendron chinense var.glabriusculum SCHNEID BY USING ~(14)C-TRACER METHOD

Semi-plants of two-years old Phellodendron Chinense Schneid were labeled by()~(14)CO_2 CO_2 tracer.Girdled the phloem of the trunk and wrapped the barked trunk with plastic film.After ward,Specimens were made through combusting and then,the radioactivity was measured by Liquid Scintillation Counting(LSC).The results showed that the carrying speed for(()~(14)C-)assimilates of non-girdled phloem were 52.0cm/h which making it possible to carry assimilates from crown to the root system in about 3 hours.It were also indicated the distributing law for()~(14)C-assimilates of the crown leaves: for the non-girdled,the middle leaves of the crown were highest,bottom lowest and for the girdled,the top became highest while the bottom remained to be unchanged.Additionally,on the 15th day,()~(14)C-assimilates were detected in the regeneration phloem and the parts beneath the barking position as well as the root system that indicated the physiological function of regenerated phloem had recovered.