N-乙酰半胱氨酸对山羊子宫内膜基质细胞增殖及相关基因表达的影响

旨在探究N-乙酰半胱氨酸(N-acetyl-L-cysteine,NAC)对山羊子宫内膜基质细胞(goat endometrial stro-mal cells,gESCs)的影响.本研究以山羊子宫内膜基质细胞为对象,体外培养基中添加浓度梯度分别为100、200、400μmol·L-1的NAC,将0μmol·L1 NA...

The Effect of N-acetylcysteine on Proliferation and Related Gene Expression of Goat Endometrial Stromal Cells in vitro

The aim was to explore the effect of N-acetyl-L-cysteine(NAC)on goat endometrial stromal cells(gESCs).In this study,goat endometrial stromal cells were used as the object.Three concentration gradients of 100,200,400μmol·L^-1NAC were added to the culture medium.The 0μmol·L^-1NAC was regarded as the blank group and CCK-8 method was used to screen the optimal concentration of NAC and judge its effect on cell proliferation.Then DCFH-DA probe and RT-qPCR were used to detect the optimal concentration of NAC on the intracellular reactive oxygen content,cell cycle and proliferation related factors,and goat reproductive performance-related genes TGF-β1 and TGF-β3 mRNA expression levels.The results showed that the concentration of 200μmol·L^-1NAC had a more significant effect on the proliferation of goat endometrial stromal cells than other gradients.In addition,200μmol·L^-1NAC reduced the ROS content of cells compared with the blank group(P<0.01),thereby delaying cell senescence.RT-qPCR results showed that the addition of 200μmol·L^-1NAC significantly increased the expression levels of cell cycle and proliferation-related factors CyclinA1,CyclinE,PCNA mRNA(P<0.01),and reduced the mRNA expression of cell cycle factor CyclinD2(P<0.01);and extremely significantly reduced the TGF-β1 gene(P<0.01)of the transforming growth factor family and significantly reduced the mRNA expression level of the TGF-β3 gene(P<0.05).Therefore,this study speculates that 200μmol·L^-1NAC may promote cell proliferation by regulating the expression levels of CyclinA1,CyclinE,PCNA,and CyclinD2 in cells,and affect goat reproduction traits by regulating the expression levels of transforming growth family factors TGF-β1 and TGF-β3.This study provide a theoretical basis for further research on the mechanism of NAC’s influence on goat reproductive traits.