栽培一枝蒿粗多糖佐剂增强口蹄疫疫苗的皮下免疫效果

旨在评价新疆栽培一枝蒿粗多糖(cultivated A rtemisia rupestris L.crude polysaccharides,CARCP)对口蹄疫灭活疫苗(foot and mouth disease vaccine,FMDV)的免疫佐剂活性,确定多糖佐剂的有效性.选用不同剂量的CARCP与FMDV配伍...

Immuno-enhancement Effects of Cultivated Artemisia rupestris L.Crude Polysaccharide on Foot-and-mouth Disease Vaccine via Subcutaneous Route

The purpose of this study was to evaluate the adjuvant effects of cultivated Artemisia rupestris L.crude polysaccharides(CARCP)on inactivated foot-and-mouth disease vaccine(FMDV).ICR mice were subcutaneously administrated with different doses of CARCP combined with FMDV.ELISA was used for the detection of FMDV-specific antibodies.T cell subsets in spleen and lymph nodes,and the expression of dendritic cell surface molecules as well as regulatory T cells(Tregs)in spleen were detected by flow cytometry.Mice were weighed every week,and observed the growth condition of mice after immunization.Compared with FMDV alone,higher levels of FMDV-specific IgG,IgG1 and IgG2a were induced by both medium-dose and high-dose of CARCP(P<0.01).Medium-dose of CARCP not only significantly increased the percentage of CD4,CD8,and CD44 T cells in the spleen and lymph nodes of mice(P<0.05),but also promoted the expression of CD86 and MHC-II in DC(P<0.05),whereas the CD4⁺CD25⁺Foxp3⁺T cells were significantly down-regulated(P<0.05).There was no noticeable influence on the growth and body weight of immunized mice(P>0.05).The results suggested that CARCP as an adjuvant for FMDV could augment the specific humoral and cellular immune responses via inducing DC maturation,inhibiting Tregs-level,which provided a basis for the development of polysaccharide adjuvant of FMDV.