三种炎症因子在致炎大鼠乳腺组织中的表达

为探明在酯多糖（LPS）致炎的大鼠乳腺中,黏附因子-1（ICAM-1）、肿瘤坏死因子-α（TNF-α）、白介素-1β（IL-1β）表达分布的变化。取产后5 d乳房注射LPS致炎大鼠的乳腺组织,及LPS致伤的体外培养大鼠乳腺MVECs（Microvascular Endothelial Cells）,采用免疫组化方法检测ICAM-1、TNF-α和IL-1β的表达。结果表明：LPS处理大鼠乳腺后4~24 h内,乳腺组织切片试验和体外培养大鼠乳腺微血管内皮细胞（Rat MammaryMicrovascular Enclothelial Cells,RMMVECs）试验中ICAM-1和IL-1β的表达逐渐增强,TNF-α在乳腺组织切片试验中6 h表达显著增强,之后则逐渐减弱。在体外培养RMMVECs中-α表达逐渐增强。表达部位主要在乳腺上皮细胞和血管内皮细胞,IL-1β在腺泡腔中的白细胞中也有表达。由此可见,LPS处理大鼠乳腺后ICAM-1、TNF-α和IL-1β这三种细胞因子均参与了乳腺炎症过程中的免疫反应。

Expression of three cytokines in rat inflammatory mammary tissue

Objective: This experiment was conducted to study the expression of ICAM-1,TNF-α and IL-1β during LPS-induced rat mastitis.Method: LPS infected mammary gland of SD rat on the 5th day of lactation and RMMVECs(Rat Mammary Microvascular Endothelial Cells) in vitro were prepared for immunohistochemistry analysis of ICAM-1,TNF-α and IL-1β.Result: A positive expression of ICAM-1,TNF-α and IL-1β in mammary gland epithelial cells and mammary gland endothelial cells was detected after LPS stimulating,IL-1β either expressed in acinar lumina.In mammary tissue experiment,expression of ICAM-1,TNF-α and IL-1β were strongly up-regulated at 6 h,then the level of ICAM-1 and IL-1β were increased,but the level of TNF-α was decreased.In RMMVECs experiment,expression of ICAM-1,TNF-α and IL-1β were increased gradually.Conclusion: The results indicated that ICAM-1,TNF-α and IL-1β involved in immune response in LPS-induced mastitis.