六种中药生物碱对LPS作用下猪血管内皮细胞分泌细胞因子的影响

通过研究6种中药生物碱对细菌内毒素（LPS）作用下猪血管内皮细胞分泌细胞因子血栓素B2（TXB2）,内皮素-1（ET-1）和E-选择素（E-selectin）的影响,拟探讨生物碱抗LPS的药理作用机制。培养猪血管内皮细胞至单层融合状态,加入LPS刺激,3 h后加入高、中、低3种浓度的6种中药生物碱青藤碱、防己诺林碱、水苏碱、川芎嗪、苦参碱和吴茱萸碱,继续培养21 h后收集细胞上清液,用ELISA测定TXB2,ET-1和E-selectin的含量。结果,水苏碱高剂量组和川芎嗪高剂量组的TXB2含量极显著低于LPS对照组,川芎嗪中剂量组和吴茱萸碱高剂量组显著低于LPS对照组;苦参碱高剂量组的ET-1含量极显著低于LPS对照组,青藤碱高剂量组显著低于LPS对照组;防己诺林碱高剂量组和吴茱萸碱中剂量组的E-selectin含量显著低于LPS对照组。结果提示六种中药生物碱具有明显的抗细菌内毒素作用,可在由TXB2,ET-1和E-selectin介导的机体凝血、休克和炎症等病理损伤过程中发挥治疗作用。

Six alkaloids inhibit secretion of TXB2, ET-1 and E-selectin in LPS-induced porcine iliac artery endothelial cells

The research was to investigate the antiendotoxin action and mechanism of Sinomenine,Fangchinoline,Stachydrine,Chuanxionggzine,Oxymartrine and Evodiamine.Endothelial cells were challenged with 1 μg/mL LPS for 3 h,then treated separately with six alkaloids at three concentrations(1,5 and 10 μg/mL).The cells were incubated at 37 ℃ in a cell incubator for 21 h.The supernatants were collected and analyzed the contents of TXB2,ET-1 and E-selectin by ELISA kits.The results showed that as compared with LPS control group,the TXB2contents were significantly decreased in the high-dose group of Stachydrine,the middle-and high-dose groups of Chuanxionggzine,and the middle-dose group of Evodiamine;The ET-1 contents were significantly decreased in the high-dose group of Sinomenine,and the high-dose group of Oxymartrine;The E-selectin contents were significantly decreased in high-dose group of Fangchinoline,and the middle-dose group of Evodiamine.It suggested that six alkaloids might exert beneficial therapeutic effects on thromboxane,shock and inflammation via these cytokines.