1株高温型厌氧产氢细菌的分离与鉴定

利用平面夹层和平板培养瓶厌氧法从油田区的岩石中分离到8株细菌,经气体组成及产氢能力检测,发现其中2株细菌具有产氢能力。在60℃高温条件下,菌株ZX-1的氢转化率最高,为1.8mol H2.mol-1葡萄糖。该菌为革兰氏阴性、杆菌、有芽孢、无鞭毛,菌体大小为(0.35～0.45)μm×(2～8)μm;菌落特征表现为圆形、乳白色、表面光滑、不透明;生理生化试验结果表明,明胶液化、硝酸盐还原、柠檬酸盐利用、葡萄糖为阳性,氧化酶、淀粉水解、V-P反应为阴性,初步鉴定为短芽孢杆菌属(Brevibacillus)。经16S rDNA PCR扩增及测序结果表明,该菌序列与Brevibacillus borstelensis的序列相似性达100%,进一步确定该菌为波茨坦短芽孢杆菌。

Isolation and Identification of an Anaerobic Bacteria with Thermophilic Hydrogen Production

Eight stains were isolated from rocks in oilfield by the way of interlayer culture and anaerobic bottle.Two strains could produce hydrogen detected by gas chromatograph and ability.ZX-1 strain had the highest yield of hydrogen which was 1.8mol H2·mol-1 glucose at 60℃.It was 0.35μm~0.45μm×2μm~8μm,bacilliform sharp without flagellum,gram-negative and with spore.The colonies of ZX-1 were round,creamy,ganoid,opaque.They had smooth surface,not transparent.The results of physiological biochemical test showed that gelatin liquefaction,nitrate reduction test,citrate utilization and glucose were positive,and oxidase reaction,starch hydrolysis and VP reaction were negative.The result of comparing its 16S rDNA sequence with known seguence showed that it was identified as Brevibacillus borstelensis.