| 食品动物源沙门菌oqxAB基因的检测及水平传播机制研究 |
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| 引用本文： | 刘继,宋立,魏飞龙,汤电,张静远,张小华,李健,蒋红霞.食品动物源沙门菌oqxAB基因的检测及水平传播机制研究[J].华南农业大学学报,2011,32(4):105-109. |
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| 作者姓名： | 刘继 宋立 魏飞龙 汤电 张静远 张小华 李健 蒋红霞 |
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| 作者单位： | 1. 华南农业大学兽医学院,广东省兽药研制与安全评价重点试验室,广东广州510642
2. 中国兽药监察所,北京,100081 |
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| 基金项目： | 国家自然科学基金(30871890); 国家自然科学基金-广东省自然科学基金联合基金重点项目(U0631006) |
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| 摘 要： | 为了调查食品动物源沙门菌Salmonella oqxAB耐药基因的流行情况和水平传播机制,采用二倍琼脂稀释法测定临床分离的31株沙门菌对常用抗菌药物的敏感性,PCR方法特异扩增耐药基因oqxAB,并对扩增产物克隆测序.通过接合转移试验验证oqxAB基因的水平传播.试验结果表明,31株沙门菌对氨苄霉素的耐药率最高,达64...
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| 关 键 词： | 沙门菌 oqxAB基因 最低抑菌浓度 质粒 接合 |
Detection and Dissemination of oqxAB Gene Among Salmonella Isolates from Food-Producing Animals |
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| LIU Ji,SONG Li,WEI Fei-long,TANG Dian,ZHANG Jing-yuan,ZHANG Xiao-hu,LI Jian and JIANG Hong-xia.Detection and Dissemination of oqxAB Gene Among Salmonella Isolates from Food-Producing Animals[J].Journal of South China Agricultural University,2011,32(4):105-109. |
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| Authors: | LIU Ji SONG Li WEI Fei-long TANG Dian ZHANG Jing-yuan ZHANG Xiao-hu LI Jian and JIANG Hong-xia |
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| Institution: | LIU Ji1,SONG Li2,WEI Fei-long1,TANG Dian1,ZHANG Jing-yuan1,ZHANG Xiao-hua1,LI Jian1,JIANG Hong-xia1(1 Guangdong Key Lab for Veterinary Drug Development and Safety Evaluation,College of Veterinary Medicine,South China Agricultural University,Guangzhou 510642,China,2 China Institute of Veterinary Drug Control,Beijing 100081,China) |
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| Abstract: | Susceptibilities of total 31 Salmonella isolates were determined by agar dilution method.oqxAB gene was amplified among 31 Salmonella isolates using specific primers.PCR products were cloned into a vector pMD19-T for sequencing.Conjugation tests were conducted among oqxAB positive Salmonella using Escherichia coli J53AziR as a recipient.The susceptibilities of 31 Salmonella isolates for common use antimicrobials showed that the resistant occurrence for ampicillin was ranked the highest(64.5%),42%-55% for am... |
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| Keywords: | Salmonella oqxAB gene minimum inhibitory concentration plasmid conjugation |
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