Nuclear genome constitution and other characteristics of somatic hybrids between dihaploid Solanum acaule and tetraploid S. tuberosum |
| |
Authors: | Tetsuya Yamada Kazuyoshi Hosaka Kumi Nakagawa Noriyo Kaide Shuji Misoo Osamu Kamijima |
| |
Institution: | (1) Division of Science of Biological Resources, Graduate School of Science and Technology, Kobe University, Nada, Kobe 657, Japan;(2) Present address: Department of Biotechnology, National Institute of Agrobiological Resources, Kannondai, Tsukuba, Ibaraki 305, Japan;(3) Experimental Farm, Kobe University, 1348 Uzurano, Kasai, Hyogo 675-21, Japan;(4) Laboratory of Plant Breeding, Faculty of Agriculture, Kobe University, Nada, Kobe 657, Japan |
| |
Abstract: | Eleven somatic hybrids (2n = 68 to 74) obtained between S. tuberosum ssp. tuberosum cv. Dejima (2n = 48) and ATDH-1 (2n =
24), an anther-culture-derived dihaploid of S. acaule (Yamada et al., 1997), were characterized by nuclear RFLP markers using
49 single-copy DNA probes distributed throughout the potato genome (2 to 6 probes per chromosome). One of the somatic hybrids,
DA8-2, had 72 chromosomes and all the Dejima- and ATDH-1-specific markers (124 and 103 bands, respectively), suggesting the
presence of a whole set of both parental chromosomes. The other somatic hybrids lost varying numbers of markers up to seventeen.
The pattern of the loss of markers indicated the elimination of five chromosomes among four somatic hybrids. A nucleolar organizer
region of chromosome 2 was often eliminated in the somatic hybrids. The somatic hybrids studied here had higher frequencies
of multivalent formation than the S. tuberosum parent. They had reasonably good seed set when pollinated with S. tuberosum
pollen. Hence, homoeologous recombination between S. acaule and S. tuberosum chromosomes is possible and useful traits from
S. acaule may be transferred to the S. tuberosum gene pool.
This revised version was published online in July 2006 with corrections to the Cover Date. |
| |
Keywords: | chromosome elimination pollen fertility RFLP markers Solanum acaule Solanum tuberosum somatic hybrids |
本文献已被 SpringerLink 等数据库收录! |
|