均匀设计对大肠杆菌产谷氨酸脱羧酶培养基优化的应用

为提高大肠杆菌单位发酵液中谷氨酸脱羧酶的产量,采用均匀设计,对培养基组成与发酵条件等9因素进行优化,后续分析以逐步线性回归、偏最小二乘回归建模并推论最优组合,结果表明:1)基于逐步线性回归模型,最优组合酶活性预测值(1.611)高于实测值(1.263),表明各因素与酶活性间更多地呈现非线性关系.2)基于二次多项式偏最小二乘回归和二次多项式偏最小二乘回归(考虑互作项)获得的最优组合相同,但酶活性预测值(2.090)高于实测值(0.927);基于二次多项式偏最小二乘回归(考虑平方项)获得的最优组合预测值(1.920)与实测值(1.933)接近.显示各因素与酶活性更多地呈现二次曲线关系,但各因素间的交互作用并不明显.3)基于均匀设计和二次多项式偏最小二乘回归(考虑平方项),仅经2轮共23次试验,即高效筛选得一满意组合:牛肉膏8 g/L,蛋白胨15 g/L,NaCl 2.79 g/L,谷氨酸 0.5 g/L,葡萄糖3 g/L,磷酸二氢钾4 g/L,硫酸镁0.56 g/L,pH6.87,发酵时间18 h;实测酶活性1.933,高于初始组合的酶活性(1.528).

Application of uniform design method in medium optimization for glutamate decarboxylase

To improve the production of GAD in per unit of medium,uniform design method was used to optimize the medium in nine factors with satisfactory results. 1) Based on the stepwise regression,it indicated that all factors and the dependent OD630 are nonlinear, the actual test values are OD630(0.927),the predication values are OD630(1.611).2) Based on the Partial Least Square Regression(PLS) and the Partial Least Square Regression (mutual)it indcated that the actual test values OD630(1.263) are not fit for the p...