Vitrification of Bovine Oocytes by Open Pulled Straw |
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Authors: | ZHU Shi-en ZENG Shen-ming WU Tong-yi MENG Qing-gang ZHANG Zhong-cheng CHEN Yong-Fu |
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Institution: | 1. College of Animal Science and Technology 2. College of Biological Science, China Agricultural University, Beijing 100094, P. R. China |
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Abstract: | Bovine oocytes cultured in vitro for 6 hours or 22 hours were cryopreserved in different vitrification solutions (EFS40, EFS50, EDFS30 or EDFS40) by the two-step method with OPS (open pulled straw).The best results were achieved by using EDFS30 to cryopreserve the oocytes either for in vitro fertilization or for chemical activation. The blastocyst rates were 12% and 17% in 6 hour and 22 hour cultures respectively following in vitro fertilization. If frozen-thawed oocytes were continued in culture up to 24 hours, and were activated by chemicals, the blastocyst rates were 22% and 24% in 6-hour and 22-hour groups respectively.There were no statistical differences between frozen and fresh oocytes (P > 0.05). |
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Keywords: | OPS Vitrification Oocyte Bovine |
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