中草药对牙鲆免疫力的影响

以初始体重为(22.89±12.60)g的牙鲆幼鱼为试验对象,在室内控温循环水槽中进行中草药对牙鲆免疫力影响的试验.向基础饲料中添加的中草药含量分别为1%、3%和5%,经过15、30和45 d后分别取血清进行免疫指标的分析测定,取鱼表皮进行常规组织学制片,在光学显微镜下观察粘液细胞的数量变化.饲喂实验结束时通过背部肌肉...

Effect of Chinese herbal medicine on immunity of flounder Paralichthys olivaceus

Flounder (Paralichthys olivaceus) juveniles,(22.89±12.60)g,were stocked in circulating water and temperature controlled channels and fed the diets containing 1%,3% and 5% Chinese herbal medicine.On the 15th,30th and 45th day,serological immunological index were analyzed.Histology section of epithelium and the number of mucus cells were observed under microscope.Noxious experiment with injecting Edwardsiella Tarda into the back muscle was carried.Results showed that the number of white blood cells,the active of lysozyme,alkaline phosphatasein and the total protein in serum were increased as the inrising of concentration of Chinese herbal medicine and the sustained time under the condition of 3% and 5%.Complement C3 and superoxide dismutase were increased after feeding the diets containing 5% Chinese herbal medicine.Numbers of mucus cells in the skin,the index of spleen and rate of the immune protection were positive correlated with the concentration of Chinese herbal medicine and sustained time.The rate of immune protection was as high as 80% in 5% concentration group.It showed that feeding Chinese herbal medicine added in could improve the flounder’s immunity.