绵羊腹腔镜、开腹皱胃瘘管手术对应激反应的影响

通过检测绵羊术后白介素-6(Interleukin-6,IL-6),C反应蛋白(C-reactive protein,CRP)和肿瘤坏死因子(Tumor necrosis factor,TNF-α)的变化比较传统开腹皱胃瘘管手术(Open abomasal fistula surgery,OAF)与腹腔镜皱胃瘘管手术(Laparoscopic abomasal fistula surgery,LAF)对应激反应的影响。12只健康绵羊分别进行开腹、腹腔镜皱胃瘘管手术,在术前、术后即刻,1,3,5,7d清晨饲喂前颈静脉采集血液样本,分离血清。应用ELASI方法进行IL-6,CRP和TNF-α的检测。结果表明,LAF、OAF两组的IL-6、CRP和TNF-α与术前相比,都有显著性差异(P<0.05)。与LAF组比较,OAF组CRP术后即刻和术后1、3d差异显著(P<0.05),IL-6术后1d差异显著(P<0.05),TNF-α术后1d差异极显著(P<0.01)。说明LAF和OAF都可导致血清中CRP、IL-6、TNF-α的短暂性升高,但OAF较LAF对IL-6、CRP和TNF-α的影响更为显著,即开腹皱胃瘘管手术较腹腔镜手术对机体应激反应的影响更大。

Effect of laparoscopic and open abdominal abomasal fistula surgery on stress reaction in sheep

Comparison the effect of laparoscopic and open abdominal abomasal fistula surgery on stress reaction by detecing IL-6,CRP and TNF-a in sheep.12 healthy sheep were divided randomly into two groups:open abomasal fistula surgery and laparoscopic abomasal fistula surgery.The jugular vein blood samples were obtained at the time of the preoperative day,and immediately,1,3,5,7 after operations before the morning feeding.Serum were centrifugated,then IL-6,CRP and TNF-α were detected by the method of ELASI.The results showed that LAF,OAF groups of IL-6,CRP and TNF-α,compared with the preoperative,there were remarkable differences (P<0.05).The degree in the changes of CRP between LAF and OAF were remarkable difference (P<0.05) immediately after operation and1,3 d after operation,IL-6 1 d after operation were remarkable difference (P<0.05),TNF-α were significantly different in 1 d after operation (P<0.01).This experiment indicated that both LAF and OAF can lead CRP,IL-6,TNF-α increased in the short time,but compared with LAF,the effect of OAF on IL-6,CRP and TNF-α were more significant,that was,the open abomasal fistula surgery had obviously effect on stress reaction in sheep than the laparoscopic surgery.