| 转抗虫基因三系优良保持系的获得 |
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| 引用本文： | 马炳田,王玲霞,李平,朱祯,周开达.转抗虫基因三系优良保持系的获得[J].作物学报,2004,30(1):60-65. |
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| 作者姓名： | 马炳田 王玲霞 李平 朱祯 周开达 |
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| 作者单位： | 四川农业大学水稻研究所, 四川温江611130 |
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| 基金项目： | 国家高技术研究发展计划(863计划)，四川省生物技术攻关项目，四川省科技攻关项目 |
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| 摘 要： | 以质粒pBUSCK-HPT作抗虫基因供体，优良籼型保持系D62B为受体，采用基因枪转化法，获得了转抗虫基因sck的植株。将转基因保持系与不育系D62A回交，获得转抗虫基因不育系。分子证据表明，外源基因能稳定转移到不育系中。蛋白活性测定显示，转基因在不育系中得到表达。本文还讨论了转基因技术在水稻育种中的作用。
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| 关 键 词： | 水稻 籼稻 抗虫性 转基因 修饰的豇豆胰蛋白酶抑制剂基因 |
| 收稿时间： | 2002-07-22 |
| 修稿时间： | 2002年7月22日 |
Obtaining Transgenic Elite Indica Maintainer Line Containing Modified CpTI(sck) |
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| MA Bing Tian ,WANG Ling Xia ,LI Ping ,,ZHU Zhen ,ZHOU Kai Da.Obtaining Transgenic Elite Indica Maintainer Line Containing Modified CpTI(sck)[J].Acta Agronomica Sinica,2004,30(1):60-65. |
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| Authors: | MA Bing Tian WANG Ling Xia LI Ping ZHU Zhen ZHOU Kai Da |
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| Institution: | Rice Research Institute of Sichuan Agricultural University, Wenjiang 611130, Sichuan |
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| Abstract: | The transgenic D62B, an elite indica maintainer line, containing modified CpTI (sck) , was obtained by bombardment With the transgenic D62B as donor, D62A containing sck was developed PCR and Southern blotting analyses indicated that sck gene could be delivered to CMS line stably The field performance of transgenic plants was similar to the non transgenic control, but the resistance to insects was increased The use of genetic transformation in rice breeding was also discussed |
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| Keywords: | Oryza sativa L Indica Resistance to insects Transgene Modified CpTI (sck) |
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