| 羊驼皮肤KAP3.2基因3′端序列的cDNA克隆及序列分析 |
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| 引用本文： | 刘一飞,范瑞文,董常生.羊驼皮肤KAP3.2基因3′端序列的cDNA克隆及序列分析[J].山西农业科学,2010,38(7):108-111. |
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| 作者姓名： | 刘一飞 范瑞文 董常生 |
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| 作者单位： | 1. 山西农业大学动物科技学院,羊驼生物工程实验室,山西,太谷,030801;山西省农业科学院畜牧兽医研究所,山西,太原,030032
2. 山西农业大学动物科技学院,羊驼生物工程实验室,山西,太谷,030801 |
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| 基金项目： | 国家"948"项目，国家自然科学基金项目 |
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| 摘 要： | 利用大规模筛选表达序列标签(ESTs)方法,从羊驼皮肤cDNA文库中筛选出角蛋白关联蛋白(Ker-atin Associated Protein,KAP)KAP3.2基因的3′端序列,Genbank序列号为EV554632。利用生物信息学方法对此序列进行分析,结果表明,该基因片段大小为454bp,完整的ORF编码35个氨基酸;通过对其功能域分析得出,不含信号肽,在系统进化上,羊驼与人和黑猩猩的遗传距离较近。
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| 关 键 词： | 羊驼 KAP3.2 序列分析 |
The Clone and Sequence Analysis of KAP3.2 Gene 3'End cDNA in Alpaca |
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| LIU Yi-fei,FAN Rui-wen,DONG Chang-sheng.The Clone and Sequence Analysis of KAP3.2 Gene 3'End cDNA in Alpaca[J].Journal of Shanxi Agricultural Sciences,2010,38(7):108-111. |
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| Authors: | LIU Yi-fei FAN Rui-wen DONG Chang-sheng |
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| Institution: | 1.Laboratory of Biology Engineering in Alpaca,College of Animal Sciences and Technology,Shanxi Agricultural University,Taigu 030801,China;2.Institute of Animal Husbandry and Veterinary,Shanxi Academy of Agricultural Sciences,Taiyuan 030032,China) |
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| Abstract: | Using large scale screening ESTs,the keratin associated protein （KAP）3.2 gene 3＇end were screened from the alpaca skin cDNA library and the accession number is EV554632.We analyzed the bioinformatics of this sequence.KAP3.2 gene 3＇end,whose size was 454 bp with complete ORF which encoded 35 aminoacids and the putative protein had no signal peptide.According to the homologue analysis among mammalians,the alpaca have the nearest distance with human and pan trqlodytes. |
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| Keywords: | KAP3 2 |
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