| 稻瘟病菌病组织与单孢菌株的分子指纹分析比较研究 |
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| 引用本文： | 汪安云. 稻瘟病菌病组织与单孢菌株的分子指纹分析比较研究[J]. 云南农业大学学报(自然科学版), 2000, 15(1): 24-26 |
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| 作者姓名： | 汪安云 |
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| 作者单位： | 云南省烟草研究院农业研究所高新室!昆明650106 |
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| 摘 要： | 通过分析来自1999年云南省石屏县的5个稻瘟病菌标样，比较直接从病组织提取的DNA与其相应的单孢菌株提取的DNA，它们的rep-PCR分子指纹相同，表明同一病组织上的水稻稻瘟病菌无遗传上的差异。从而可以直接从病组织中提取DNA，省去单孢分离、培养等繁杂劳动，缩短了从病标样采集到指纹分析的时间，可极早地检测当年稻瘟病菌的遗传结构变化规律，为及时防治稻瘟病提供可靠的科学依据。
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| 关 键 词： | 稻瘟病菌 rep-PCR 分子指纹 |
| 收稿时间： | 1999-09-09 |
The Comparison of Molecular Fingerprint from the Diseased Neck Tissue with Corresponding Isolates |
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| Wang Anyun. The Comparison of Molecular Fingerprint from the Diseased Neck Tissue with Corresponding Isolates[J]. Journal of Yunnan Agricultural University, 2000, 15(1): 24-26 |
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| Authors: | Wang Anyun |
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| Affiliation: | (Laboratory of Advanced Science and Technology, Yunnan Academy of Tobacco Science, Kunming 650106) |
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| Abstract: | The study compared the fingerprints by using rep PCR from the diseased neck tissue with corresponding isolates. The results showed that they had the same fingerprints, and demonstrated that those isolates from the same diseased tissue had no genetic difference, or from extracted DNA take from the diseased tissue directly. |
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| Keywords: | Blast isolates rep PCR Moleculor fingerprint |
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