甘蓝型油菜胚胎离体培养体系的建立及其在油脂合成积累研究中的应用

为便于体外研究甘蓝型油菜的胚胎发育和种子油脂积累规律，以春油菜862的胚胎为材料，构建胚胎离 体培养体系，通过综合比对基础培养基类型、蔗糖浓度、琼脂浓度，确定含蔗糖8%和琼脂0.6%的B5培养基作为胚 胎离体培养和胚胎胚性生长的最佳体外培养体系，并用于研究种子油脂的积累和变化。在体系中分别加入甘蓝型 油菜的角果皮和叶片提取物，调查对胚胎油脂积累的影响，发现含有角果皮提取物和叶片提取物的培养基均能提 高胚胎油脂积累效率，且角果皮提取物对胚胎油脂积累的效率显著高于叶片提取物，源于高油材料的角果皮、叶片 提取物对胚胎油脂的积累效率显著高于低油材料。

Establishment of an in vitro embryonic growth system and its application in rapeseed oil synthesis and accumulation study

To facilitate the study on embryo developrnent and fatty acid accumulation, tissue culture of rape seeds were carried out by using Brassica napus spring type 862. Study of influence factors on 862 oil synthesis and accumulation showed that B5 basal medium with 8% sucrose and 0.6% agar was optimal. Extracts from pericarp and leaf of different varieties in the medium could improve oil accumulation efficiency in embryo. While pericarp extract had higher accumulation efficiency than that from leaf. Extracts of pericarp and leaf from the high oil varieties possessed much higher oil accumulation efficiency.