| CRISPR/Cas9介导的多基因编辑系统在大豆中的应用 |
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| 引用本文： | 关贝贝,王燕娟,陈海峰,陈水莲,沙爱华,曹东. CRISPR/Cas9介导的多基因编辑系统在大豆中的应用[J]. 中国油料作物学报, 2021, 43(1): 149-160. DOI: 10.19802/j.issn.1007-9084.2020305 |
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| 作者姓名： | 关贝贝 王燕娟 陈海峰 陈水莲 沙爱华 曹东 |
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| 作者单位： | 1. 长江大学农学院，湖北荆州，434025；2. 中国农业科学院油料作物研究所，湖北武汉，430062 |
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| 基金项目： | 国家自然科学基金面上项目(32072097);中央级科研院所基本科研业务费专项(1610172018008)。 |
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| 摘 要： | 自CRISPR/Cas9(clustered regularly interspaced short palindromic repeats/CRISPR-associated protein 9)基因组编辑技术发现以来,迅速在作物中得到广泛应用.但是,CRISPR/Cas9多基因编辑系统在大豆中的研究尚待开发.本文利...
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| 关 键 词： | 大豆 CRISPR/Cas9 GmAS1/2 GmSHP1/2 GmSTK 多基因编辑 |
Application of CRISPR/Cas9-mediated multiplex genome editing system in soybean genome editing |
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| GUAN Bei-bei,WANG Yan-juan,CHEN Hai-feng,CHEN Shui-lian,SHA Ai-hua,CAO Dong. Application of CRISPR/Cas9-mediated multiplex genome editing system in soybean genome editing[J]. Chinese Journal of Oil Crop Sciences, 2021, 43(1): 149-160. DOI: 10.19802/j.issn.1007-9084.2020305 |
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| Authors: | GUAN Bei-bei WANG Yan-juan CHEN Hai-feng CHEN Shui-lian SHA Ai-hua CAO Dong |
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| Affiliation: | 1. College of Agriculture, Yangtze University, Jingzhou 434025, China;2. Key Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture and Rural Affairs, Oil Crops Research Institute, Chinese Academy of Agricultural Sciences, Wuhan 430062, |
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| Abstract: | To apply CRISPR/Cas9(clustered regularly interspaced short palindromic repeats/CRISPR associated protein 9)based genome editing system on soybean functional genomics and breeding,CRISPR/Cas9-mediated targeted mutagenesis were obtained for more than 5 genes in soybean.One CRISPR/Cas9 plasmid conferred 6 sgRNA expression cassettes targeting 4 Glycine max ASYMMETRIC LEAVES1(GmAS1)genes and 3 GmAS2 genes,and another plasmid conferred 8 sgRNA expression cassettes targeting 6 G.max AGAMOUS1(GmAG)genes and 5 G.max SHATTERPROOF1(GmSHP1/2)genes.From the double mutagenesis of 3 GmAS1 genes and 3 GmAS2 genes,a phenotype of upward curling leaves and shorten leaf petiole length was discovered.From the double mutagenesis of 2 GmSHP1/2 genes and 2 GmSTK genes,sterility phenotype was found. |
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| Keywords: | soybean CRISPR-Cas9 GmAS1/2 GmSHP1/2 GmSTK polygene editing |
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