转mEPSPS基因甘蓝型油菜对根际土壤细菌群落结构和多样性的影响

转基因作物外源基因的表达是否会对土壤微生物生态系统产生不利影响是转基因作物环境安全评价中的重要一环,也关系到作物新品种的应用与推广.为此,本文采用第三代16S扩增子高通量测序技术,对转mEP-SPS基因的甘蓝型油菜甲HX6及其亲本甲9707不同发育阶段的根际土壤细菌群落进行了比较分析.结果显示,样品中油菜根际的优势细菌...

Effect of transgenic Brassica napus with mEPSPS gene on diversity and structure of rhizosphere microbial communities

Evaluating possible negative effects of alien genes expressed in transgenic plants is essential in environmental safety assessment of genetically modified(GM)organisms.We compared bacterial communities in rhizosphere soil of Brassica napus overexpressing mEPSPS(JHX6)and the control line J9707.Results showed that Proteobacteria,Bacteroidetes,Acidobacteria and Actinobacteria were the dominant bacterias in the rhizosphere soil of B.napus.Neither alpha diversity analysis(e.g.Shannon and Simpson index),PCoA nor MRPP analysis had revealed significant differences between the same developmental stage of the GM rape and control line.Meanwhile,Planctomycetes was less enriched in rhizosphere soil of GM rape at flowering stage compared with that of wild type.The enrichments of few bacterial genera were significantly different at 3 developmental stages of the GM rape and wild type.The bacterial diversity and community structure were great difference among the 3 developmental stages,especially in comparison of seedling stage.In total,mEPSPS overexpression in B.napus plants caused no significant variation on bacterial community in rhizosphere soil.While long-term dynamic monitoring is still necessary.