绵羊前体脂肪细胞分化过程中HOXC8 DNA甲基化的研究

为阐明小尾寒羊HOXC8DNA甲基化程度在前体脂肪细胞分化过程中的作用,本研究以小尾寒羊皮下前体脂肪细胞为实验材料,利用生物信息学软件和亚硫酸氢盐测序(Bisulfite Sequencing PCR,BSP)分析HOXC8启动子区和第1外显子区序列的CpG岛和甲基化水平;采用qPCR技术检测在绵羊前体脂肪细胞分化过程...

DNA Methylation of HOXC8 during Ovine Preadipocyte Differentiation

The aim of this study was to investigate the effect of HOXC8 DNA methylation during the differentiation of ovine preadipocytes.In this study,ovine subcutaneous preadipocytes were used as research material.The CpG islands and methylation levels in the promoter and the first exon sequence of HOXC8 were analyzed by bioinformatics software and bisulfite sequencing PCR(BSP).The expression of HOXC8 mRNA was detected by qPCR.The results showed that there was a CpG island in the promoter region and the first exon of HOXC8 respectively.The expression level of HOXC8 before differentiation was extremely significantly higher than after differentiation in ovine preadipocyte(P<0.01).Between day 0 and day 2 of ovine preadipocyte differentiation,no significant difference of methylation level was revealed in the promoter,but there was significant difference in the methylation level of the first exon(P<0.05).The methylation level of HOXC8 first exon was extremely significantly higher than that of the promoter region(P<0.001).According to the linear regression analysis of the methylation level of HOXC8 first exon and its mRNA expression,there was a high positive correlation between them(r=0.994,P<0.0001).HOXC8 may play an important role in ovine preadipocyte before differentiation.The methylation of the first exon of HOXC8 positively regulates its expression at transcription level during the differentiation of ovine preadipocytes.