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基于代谢组学分析平菇栽培用培养料发酵过程中代谢物的变化
引用本文:刘皓皓,刘芹,崔筱,靳荣线,高玉千,李亚楠,孔维丽,裴瑞杰,邱立友. 基于代谢组学分析平菇栽培用培养料发酵过程中代谢物的变化[J]. 中国瓜菜, 2021, 0(4): 21-30
作者姓名:刘皓皓  刘芹  崔筱  靳荣线  高玉千  李亚楠  孔维丽  裴瑞杰  邱立友
作者单位:河南农业大学生命科学学院;河南省农业科学院植物营养与资源环境研究所;河南省新乡市农业科学院食用菌研究所;南阳农业职业学院
基金项目:河南省重大公益专项(201300110700);河南省重点研发与推广专项(科技攻关)项目(212102110133);河南省现代农业产业技术体系专项资金资助项目(S2013-09,S2013-09-G02)。
摘    要:为了解发酵培养料制备过程中微生物代谢产物的组成和变化规律,采用非靶向代谢组学技术,检测分析发酵2、4、6、8和10 d时培养料的代谢组数据.结果表明:在正离子(POS)模式下,分别在T2 vs T1、T3 vs T2、T4 vs T3和T5 vs T4中筛选得到331、145、161和115种差异代谢物;在负离子(NE...

关 键 词:平菇  发酵料  代谢组学  差异代谢物

Metabonomics analysis revealed the changes of metabolites in fermentation materials for preparation of cultivation medium for Pleurotus ostreatus
LIU Haohao,LIU Qin,CUI Xiao,JIN Rongxian,GAO Yuqian,LI Yanan,KONG Weili,PEI Ruijie,QIU Liyou. Metabonomics analysis revealed the changes of metabolites in fermentation materials for preparation of cultivation medium for Pleurotus ostreatus[J]. China Cucurbits And Vegetables, 2021, 0(4): 21-30
Authors:LIU Haohao  LIU Qin  CUI Xiao  JIN Rongxian  GAO Yuqian  LI Yanan  KONG Weili  PEI Ruijie  QIU Liyou
Affiliation:(College of Life Sciences,Henan Agricultural University,Zhengzhou 450002,Henan,China;Institute of Plant Nutrition,Agricultural Resources and Environmental Science,Henan Academy of Agricultural Sciences,Zhengzhou 450002,Henan,China;Institute of Edible Mushroom,Xinxiang Academy of Agricultural Sciences,Xinxiang 453003,Henan,China;Nanyang Vocational College of Agriculture,Nanyang 473000,Henan,China)
Abstract:Non-targeted metabolomics technique was used to reveal the dynamic changes in the composition and variation of microbial metabolites in corncob composting for preparing cultivation medium of Pleurotus ostreatus.Results showed that 331,145,161 and 115 differential metabolites at POS mode were screened,including carbohydrates and derivants,amino acids,peptides,and analogues,fatty acids,vitamins,phenylpropanoids and polyketides,and other secondary metabolites,between T2 vs T1,T3 vs T2,T4 vs T3 and T5 vs T4,respectively.And 251,159,106 and 76 differential metabolites at NEG mode were screened,respectively.Furthermore,the fermentation materials contained plant growth regulators(such as indole-3-acetic acid,jasmonic acid and gibberellin)and antibacterial substances(such as chlorogenic acid,antibiotics,resveratrol).This study provided a reference for the preparation of quality-stable fermentation materials and the further understanding of the cultivation of Pleurotus ostreatus with fermentation materials.
Keywords:Pleurotus ostreatus  Fermentation material  Metabolomics  Differential metabolites
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