反相高效液相色谱法测定火龙果有机酸

摘 要 采用高效液相色谱法测定火龙果有机酸种类和含量，结果表明，色谱柱：C18（3.9mm×300mm，10um）；流动相0.04mol/L KH2PO4-H3PO4缓冲溶液：甲醇=95：5，pH2.4；流速：0.8mL/min；柱温：30℃；检测波长：214nm的检测条件下，火龙果有机酸得到良好分离。草酸、酒石酸、丙酮酸、乳酸、柠檬酸、琥珀酸、富马酸标准曲线的相关系数达0.99以上，平均回收率在90.2～105.5%，检测限分别为2.0、1.5、0.2、6.0、10.0、5.0和0.02mg/L, 各组分保留时间以及峰面积的CV（n=3）均小于1.0%，其中保留时间的日内重现性CV（n=3）＜0.4%，保留时间的日间精密度CV（n=10）＜0.2%。

Determination of Some Organic Acids in Pitaya by Reversed Phase High Performance Liquid Chromatography

A method with UV detector was established to determine the organic acids in pitaya. The chromato- graphic conditions were C18（3.9 mm × 300 mm,10 μm）used for column（at 30 ℃）with 0.04 mol/L KH2PO4-H3PO4（pH2.4）as mobile phase, and flow rate 0.8 ml/min, detector UV 214nm. The correlation coeffi- cients of calibration graphs of Oxilic acid, Tartaric acid, Pyruvic acid, lactic acid, citric acid, succinic acid and fumaric acid were above 0.99, and the averaged recoveries were 90.2%-105.5%. The limits of detection were 2.0, 1.5, 0.2, 6.0, 10.0, 5.0 mg/L and 0.02 mg/L respectively. The coefficient of variation （CV） for repeat- ability （n=3） of retention time and peak area was within 1.0%, respectively, and the CV for within-day repeat- ability （n=3） and between-day precision （n=10） of retention time was within 0.4% and 0.2% respectively.