新疆早熟棉品种SSR指纹图谱构建与品种鉴别

以新疆自1978年以来审定命名的42个早熟棉品种为材料,利用SSR分子标记对新疆早熟棉品种进行研究。从2300对SSR引物中筛选出了52对具有稳定多态性的引物,每对引物可检测到3～24个多态性片段,共检测到506个,平均9.7个,片段大小介于100～2000bp之间。对所得到的52个SSR指纹图谱进行组合和综合分析,用其中2对就可将所有供试品种区分开来。同时,分别对42个早熟棉品种进行指纹分析,获得各个品种的特异性指纹,可为今后棉花种子真伪快速鉴别奠定基础。

Construction of Fingerprinting Map Based on SSR and Identification of Cultivars for Earliness Cultivars in Upland Cotton in Xinjiang

The research on 42 earliness cultivars planted in Xinjiang was conducted, using SSR molecular markers. Among 2300 pairs of primers, 52 pairs with steady polymorphism were selected. Three to 24 polymorphic loci were detected by each pair of primers. There were 506 polymorphic fragments detected in total. The average number of polymorphic fragments detected were 9.7 per pair of primers. The band size ranged from 100 to 2000 bp. The 52 SSR fingerprint maps were combined and analyzed comprehensively, it turned out to be that all the cotton cultivars could be identified by two primer pairs. The results indicated that the SSR markers can help to construct fingerprint map with high efficiency and accuracy rapidly, and it is feasible and effective to identify cotton cultivars with SSR molecular markers. This study will lay a solid foundation for rapid authentication and quality control of cotton seeds with large sample size.