转基因741杨高效配套育苗体系建立

针对转基因741杨硬枝扦插难生根的特性，将组织培养及多种扦插繁殖技术有机结合在一起，建立了转基因741杨综合配套育苗体系，提出了适合无性繁殖的“遮阴间歇喷雾扦插”和“移棚不移苗扦插繁殖”育苗方法。转基因741杨组培快繁中，初始启动培养基为MS 6-BA1．0mg／L NAA0．1ms／L，嫩茎增殖培养基为MS 6-BA0．3～1．0mg／L IBA0～0．3mg／L，茎尖生根培养基为1／2MS 0．3IBA 1．5％蔗糖。不同月份嫩枝扦插培养的苗木中，5月份扦插的嫩枝生长较快，但苗木间分化程度较高；8月份扦插的嫩枝生长相对较慢，但苗木的分化程度低，生长整齐。不同月份苗木移栽后，生长节律相同，经过1a生长，不同月份间苗木生长差异不明显，5月份扦插的苗木生长略优于其他月份扦插的苗木。

Development of seedling cultivation system for Transgenic Hybrid Poplar 741 carrying two insect-resistant genes

Transgenic Hybrid Polar 741 is hard to root in cuttage. Tissue culture and the propagation techniques through cuttage are combined in this study to d evelopment the seedling cultivation system, and the methods of asexual seedling propag tion,cuttage using sunshade and intermittent spray and cuttage using moving canopy but not transplanting,are put forward. In the course of tissue culture for Transgenic Hybrid Poplar741, the start-up culture medium is MS + 6-BA 1.0 mg/L + NAA 0.1 mg/L, the tender stem culture medium is MS + 6-BA 0.3-1.0 mg/L+ IBA 0-0.3 mg/L, the rhizogenic culture medium is 1/2MS +0.3IBA+1.5%cane sugar.The plantlets grow fast in May but they differentiate greatly,grow comparative slowly in August but differentiate slightly and they grow identically.The growth patterns of plantlets transplanted in different month are the same.After one-year growth,the seedlings transplanted in different month have no significant difference in growth,but the seedlings transplanted in May are better than the others in growth.