| 荧光PCR法检测鸡肉中沙门氏菌的研究 |
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| 引用本文： | 贾广乐,林祥梅,韩雪清,吴绍强,刘建,梅琳.荧光PCR法检测鸡肉中沙门氏菌的研究[J].中国畜牧兽医,2007,34(2):96-97. |
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| 作者姓名： | 贾广乐 林祥梅 韩雪清 吴绍强 刘建 梅琳 |
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| 作者单位： | 中国检验检疫科学研究院，北京 100029 |
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| 摘 要： | 作者采用合成的基因探针和引物，对2株阳性标准菌株、2株野生菌株和1个无菌去离子水进行检测，结果准确可靠，整个试验过程在30 h内完成。可见，该法是一种特异、敏感、简便、快速的沙门氏菌检测方法，对快速通关将发挥积极的作用。
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| 关 键 词： | 沙门氏菌 鸡肉 荧光PCR |
| 文章编号： | 1671-7236（2007）02-0096-02 |
| 修稿时间： | 2006-06-26 |
Detection of Salmonella in Chicken with Fluorescence Polymerase Chain Reaction |
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| JIA Guang-le,LIN Xiang-mei,HAN Xue-qing,WU Shao-qiang,LIU Jian,MEI Lin.Detection of Salmonella in Chicken with Fluorescence Polymerase Chain Reaction[J].China Animal Husbandry & Veterinary Medicine,2007,34(2):96-97. |
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| Authors: | JIA Guang-le LIN Xiang-mei HAN Xue-qing WU Shao-qiang LIU Jian MEI Lin |
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| Institution: | The Chinese Academy of Inspection and Quarantine, Beijing 100029, China |
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| Abstract: | In present article,there are two positive standard salmonella bacteria,two positive sample about chicken and a sample of aseptic water.This samples are detected with fluorescence polymerase chain reaction.The results showed: The method is efficient and accurate.Furthermore,it took only 30 hours for whole PCR process.It is concluded that PCR is a specific,sensitive,rapid,simple and convenient method for detection of salmonella.It may have great values for the entry-exit inspection and quarantine. |
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| Keywords: | salmonella chicken fluorescence PCR |
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