纤维素酶解法提取剑麻总皂苷工艺研究

[目的]研究纤维素酶解法提取剑麻总皂苷工艺。[方法]采用纤维素酶解-乙醇浸提法,以熊果酸作对照品,5%香草醛-冰醋酸-高氯酸作显色剂,分光光度法测定提取物中总皂苷含量。考察酶质量浓度、酶解温度、pH、酶解时间及固液比对剑麻总皂苷提取率的影响。[结果]单因素试验显示,酶质量浓度、酶解温度、pH、酶解时间对提取率影响较大,固液比的影响较小。L9(34)正交试验结果显示,在选定的因素水平中,对剑麻总皂苷提取率影响程度为pH酶解温度酶解时间酶质量浓度。方差分析pH、酶解温度对提取率影响显著,酶解时间影响较显著。最佳工艺条件是:pH=5.5,酶解温度45℃,酶解时间100 min,酶质量浓度0.18 mg/m L,固液比1∶20,在此工艺条件下,剑麻总皂苷提取率为19.20%。[结论]该研究为纤维素酶解法提取剑麻总皂苷提供参考。

Study on Extraction Technology of the Total Sponin from Agave sisaiana Perrine by Enzymatic Hydrolysis

[Objective]To study on extraction technology of the total sponin from the Agave sisaiana Perrine by enzymatic hydrolysis. [Meth-od] The extraction technology for the total saponin from the Agave sisaiana Perrine by methods of enzymatic hydrolysis-ethanol extraction,ur-solic acid as reference substance,the contents of total saponin from Agave sisaiana Perrine was determined by 5% Vanillin-Acetic acid-perchlo-ric acid colorimetric and spectrophotometer. The influence of enzyme concentration,enzymolysis reaction temperature,pH,enzymolysis reac-tion time and solid-liquid ratio were studied on extraction rate of the total saponin. [Result] The single factor experiment results showed that, enzyme concentration,enzymolysis reaction temperature,pH,enzymolysis reaction time had greater influence,but little influence by solid-liq-uid ratio. L9(34) orthogonal experiment results showed that, in the selected factors and levels, the influence degree of extraction rate was pH > enzymolysis reaction temperature > enzymolysis reaction time > enzyme concentration. The analysis variance showed that pH and tem-perature was most significant to extraction rate of the total saponin,and then the time. The optimum extraction technology were pH 5.5,enzy-molysis reaction temperature 45 ℃,enzymolysis reaction time 100 min,enzyme concentration 0.18 mg/mL,solid-liquid ratio was 1:20,The extraction rate was 19.20% in the condition. [Conclusion] The study provided reference for the total sponin from the Agave sisaiana Perrine by enzymatic hydrolysis.