| 五味子叶枯病菌拮抗放线菌A-25-8的鉴定及发酵条件优化 |
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| 引用本文： | 王壮,雷锋杰,张爱华,傅俊范,张连学. 五味子叶枯病菌拮抗放线菌A-25-8的鉴定及发酵条件优化[J]. 安徽农业大学学报, 2018, 45(1): 149-155. DOI: 10.13610/j.cnki.1672-352x.20180302.012 |
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| 作者姓名： | 王壮 雷锋杰 张爱华 傅俊范 张连学 |
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| 作者单位： | 吉林省人参工程技术研究中心,吉林农业大学中药材学院,长春130118;吉林省人参工程技术研究中心,吉林农业大学中药材学院,长春130118;沈阳农业大学植物保护学院,沈阳110161;沈阳农业大学植物保护学院,沈阳,110161 |
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| 基金项目： | 吉林省科技发展计划项目，国家自然科学基金 |
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| 摘 要： | 经五味子根际土中分离获得1株高效拮抗五味子叶枯病菌的放线菌A-25-8,对其进行鉴定并研究其最佳发酵条件。根据菌株的形态与培养特征、生理生化特性、16SrDNA序列分析对其进行鉴定,应用牛津杯法研究A-25-8菌株抑菌活性及最优发酵条件。A-25-8菌株与Streptomyces anulatus(环圈链霉菌)的亲缘关系接近;最佳发酵条件:发酵培养基为马铃薯葡萄糖液体培养基,碳源为葡萄糖,氮源为KNO_3,发酵培养基初始pH为7.5,发酵温度为28℃,发酵时间为3 d,接种量为10%,摇床转速为160 r·min~(-1)。拮抗放线菌A-25-8鉴定为环圈链霉菌(Streptomyces anulatus),优化后A-25-8无菌发酵滤液对五味子叶枯病菌显示出更强的抑菌活性。
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| 关 键 词： | 五味子 叶枯病 拮抗放线菌 鉴定 发酵条件 |
Identification of antagonistic actinomycetes strain A-25-8 against leaf blight pathogens of Schisandra chinensis and optimization of its fermentation conditions |
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| WANG Zhuang,LEI Fengjie,ZHANG Aihu,FU Junfan and ZHANG Lianxue. Identification of antagonistic actinomycetes strain A-25-8 against leaf blight pathogens of Schisandra chinensis and optimization of its fermentation conditions[J]. Journal of Anhui Agricultural University, 2018, 45(1): 149-155. DOI: 10.13610/j.cnki.1672-352x.20180302.012 |
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| Authors: | WANG Zhuang LEI Fengjie ZHANG Aihu FU Junfan ZHANG Lianxue |
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| Affiliation: | Ginseng Engineering Technology Research Center of Jilin Province, College of Chinese Medicinal Materials, Jilin Agricultural University, Changchun 130118,Ginseng Engineering Technology Research Center of Jilin Province, College of Chinese Medicinal Materials, Jilin Agricultural University, Changchun 130118; College of Plant Protection; Shenyang Agricultural University, Shenyang 110161,Ginseng Engineering Technology Research Center of Jilin Province, College of Chinese Medicinal Materials, Jilin Agricultural University, Changchun 130118,Ginseng Engineering Technology Research Center of Jilin Province, College of Chinese Medicinal Materials, Jilin Agricultural University, Changchun 130118; College of Plant Protection; Shenyang Agricultural University, Shenyang 110161 and Ginseng Engineering Technology Research Center of Jilin Province, College of Chinese Medicinal Materials, Jilin Agricultural University, Changchun 130118 |
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| Abstract: | An actinomycetes strain A-25-8 with strong antifungal activity against leaf blight pathogens of Schisandra chinensis was isolated from the rhizosphere soil of Schisandra chinensis.Its fermentation conditions were optimized.A-25-8 was identified based on its 16S rRNA sequence,together with the morphological,physiological and biochemical characteristics.The antagonistic activity of strain A-25-8 and its optimal fermentation conditions were also investigated by Oxford plate method.As a result,A-25-8 appeared to have a close relation to Streptomyces anulatus.The optimum fermentation conditions for strain A-25-8 were as follows:PD culture medium;glucose for carbon source;KNO3 for nitrogen source;pH at 7.5;temperature at 28℃;culture time at 3 d;inoculation amount of 10％ (V/V) and rotational speed of 160 rpm.In conclusion,the antagonistic actinomycetes A-25-8 was identified as Streptomyces anulatus.A-25-8 strain shows stronger antagonistic activities against leaf blight pathogens of Schisandra chinensis under optimized fermentation conditions. |
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| Keywords: | Schisandra chinensis leaf blight antagonistic actinomycetes identification fermentation conditions |
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