大白菜3号、6号染色体双三体及其初级三体的鉴定

 利用有丝分裂染色体核型分析的方法, 鉴定同源四倍体大白菜小孢子株系940521为3 号、6 号染色体双三体; 在其与冠291 杂交的F₁代中, 利用减数分裂中期Ⅰ染色体观察的方法, 鉴定出了3 号染色体三体和6号染色体三体, 并对F₁代中的两种三体及双三体的数目进行统计, 计算出双三体中的3 号、6 号额外染色体的雌配子传递率分别为28. 26 %和47. 83 %; 利用TTC 染色方法测定3 号染色体三体、6 号染色体三体及双三体的花粉生活力, 结果表明: 3 号染色体三体、6 号染色体三体及双三体的花粉生活力差异达极显著水平。

Identification of Double Triplo-3, 6 and Acquisition of Primary Triplo-3 and Triplo-6 in Chinese Cabbage

An autotetraploid Chinese cabbage 〔Brassica campestris ssp. pekinensis (Rupr.) Olsson〕microspore line 940521 was designated as double triplo23 , 6 by mitotic chromosome karyotype analysis. By meiosis metaphase Ⅰchromosome observation , triplo23 , triplo26 and double triplo23 , 6 were identified in the F₁ of line 940521 ×Guan 291. The transmission of extra chromosomes in double triplo23 , 6 was calculated and the resultshowed that the rates of No. 3 and No. 6 chromosome were 28. 26 % and 47. 83 % respectively. The pollens of triplo23 , triplo26 and double triplo23 , 6 were stained with TTC and their activities were measured. The resultshowed that the differences of pollen activity among triplo23 , triplo26 and double triplo23 , 6 were significant at 0. 01 level .