| 外源DNA导入的大豆品种“黑生101”的RAPD初探 |
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| 引用本文： | 李希臣 雷勃钧. 外源DNA导入的大豆品种“黑生101”的RAPD初探[J]. 大豆科学, 1999, 18(3): 230-235 |
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| 作者姓名： | 李希臣 雷勃钧 |
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| 作者单位： | 黑龙江省农业科学院生物技术研究中心 |
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| 摘 要： | 黑生１０１是利用花粉管通道技术育成的一第一个大豆品种，利用１３２个随机引物对黑生１０１及其供体，受体的ＤＮＡ进行ＲＡＰＤ扩增，其中３个单引物和２个双引物组合起来，均能在后代黑生１０１中扩增出与供体相同而与受体不同的ＤＮＡ片段，证明黑生１０１中确有供体ＤＮＡ片段的导入。
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| 关 键 词： | 大豆 黑生101 RAPD 分子验证 花粉管通道 |
RAPD VERIFICATION OF THE SOYBEAN CULTIVAR "HEISHENG 101 |
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| Li Xichen Lei Bojun Lu Cuihua Qian Hua Zhou Sijun Han Yuqin Liu Zhaojun. RAPD VERIFICATION OF THE SOYBEAN CULTIVAR "HEISHENG 101[J]. Soybean Science, 1999, 18(3): 230-235 |
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| Authors: | Li Xichen Lei Bojun Lu Cuihua Qian Hua Zhou Sijun Han Yuqin Liu Zhaojun |
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| Abstract: | Heisheng 101 was the first soybean cultivar obtained from pollen tube channel. We used RAPD to amplify the total DNA of Heisheng 101 and its donor and recipient by 132 random primers. The DNA polymorphism results of RAPD molecular verification indicated :Either 3 single random primer or 2 double primers combinations can be amplified polymorphic DNA fragments from Heisheng 101. The results proved that Heisheng 101 was truly obtained from the transformation progeny of its donor DNA fragments introduced into recipient. |
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| Keywords: | Soybean Heisheng 101 RAPD Molecular verification Pollen tube channel |
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