| 用高效液相色谱法测定维生素D |
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| 引用本文： | 刘振秀,杨玉华,王秀荣,韩雅珊. 用高效液相色谱法测定维生素D[J]. 中国农业大学学报, 1991, 0(3): 29-33 |
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| 作者姓名： | 刘振秀 杨玉华 王秀荣 韩雅珊 |
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| 作者单位： | 航天医学工程研究所;北京农业大学; |
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| 摘 要： | 叙述用高效液相色谱法测定值肝油、强化食品和饲料中 VD 的方法。样品经碱性皂化、乙醚抽提、蒸发,用甲醇溶解残渣注入半制备柱(C-18柱),收集含 VD 的部分,用洗脱液再蒸发干燥,溶于异辛烷中,注入分析柱(硅胶柱)定量。本法操作简便、灵敏度高,最低可检测20IU/100g。目前的回收率范围90.1%～93.7%,平均为91.9%,重复性的标准误差是±2.9%,变异系数为±2.5%。
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| 关 键 词： | 维生素 D 高效液相色谱(HPLC) 鱼肝油 强化食品 强化饲料 |
DETERMINATION OF VITAMIN D IN FORTIFIED FOOD AND FEED BY HPLC |
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| Liu Zhenxiu Yang Yuhua Wang Xiurong (Institude of Space Medico-engineering,Beijing,Han Yashan (Beijing Agricultural University. DETERMINATION OF VITAMIN D IN FORTIFIED FOOD AND FEED BY HPLC[J]. Journal of China Agricultural University, 1991, 0(3): 29-33 |
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| Authors: | Liu Zhenxiu Yang Yuhua Wang Xiurong (Institude of Space Medico-engineering Beijing Han Yashan (Beijing Agricultural University |
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| Affiliation: | Liu Zhenxiu Yang Yuhua Wang Xiurong (Institude of Space Medico-engineering,Beijing),Han Yashan (Beijing Agricultural University) |
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| Abstract: | A high-performance liquid chromatography(t-rPLC)method for the determination of vitamin D in cod-liver oil,fortified food and feed was described. The pretreatment of sample included alkaline saponification,ether extraction and evaporation,the residue dissolued in methanol and the injected into a semiprepara- tive HPLC column (C18 reversed phase).The fraction containieg vitamin D was collected,evaporated,dried and dissolved in isooctane.The solution was quantified by injecting onto analytical HPLC column(dir... |
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| Keywords: | vitamin D high-performance liquid chromatography(HPLC) codliver oil fortified food fortified feed |
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