| Cloning and Sequence Analysis of cDNA Encoding MRJP3 of Apis cerana cerana |
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| 引用本文： | SU Song-kun ZHNEG Huo-qing CHEN Sheng-lu ZHONG Bo-xiong Stefan Albert. Cloning and Sequence Analysis of cDNA Encoding MRJP3 of Apis cerana cerana[J]. 中国农业科学(英文版), 2005, 4(9): 707-713 |
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| 作者姓名： | SU Song-kun ZHNEG Huo-qing CHEN Sheng-lu ZHONG Bo-xiong Stefan Albert |
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| 基金项目： | 国家自然科学基金，浙江省自然科学基金 |
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| 摘 要： | By screening the worker （Apis cerana cerana） heads cDNA library using a fragment of the mrjp3 gene of Apis cerana as probe, 120 positive clones were obtained. The clone containing A. cerana cerana MRJP3 （AccMRJP3） cDNA was selected. Based on the sequencing of the inserts of the positive clone, a sequence of AccMRJP3 cDNA which is 1 887 bp long including a poly （A） tail was obtained. The AccMRJP3 cDNA encompassed an open-reading frame （ORF） with 1 779 bp encoding 593 amino acids. The un-translated regions （UTR） of the 5′ end and 3′end are 46 bp and 160 bp in length, respectively. Similar to AmMRJP3 and AdMRJP3, the putative AccMRJP3 also has a repetitive region. The comparison of the repetitive region of AccMRJP3, AmMRJP3 and AdMRJP3 shows some differences between them.
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| 关 键 词： | 蜜蜂 MRJP3 基因克隆 基因序列 氨基酸 |
Cloning and Sequence Analysis of cDNA Encoding MRJP3 of Apis cerana cerana |
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| SU Song-kun,ZHNEG Huo-qing,CHEN Sheng-lu,ZHONG Bo-xiong,Stefan Albert. Cloning and Sequence Analysis of cDNA Encoding MRJP3 of Apis cerana cerana[J]. 《Agricultural Sciences in China》, 2005, 4(9): 707-713 |
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| Authors: | SU Song-kun ZHNEG Huo-qing CHEN Sheng-lu ZHONG Bo-xiong Stefan Albert |
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| Abstract: | By screening the worker (Apis cerana cerana) heads cDNA library using a fragment of the mrjp3 gene ofApis cerana as probe, 120 positive clones were obtained. The clone containing A. cerana cerana MRJP3 (AccMRJP3) cDNA was selected. Based on the sequencing of the inserts of the positive clone, a sequence of AccMRJP3 cDNA which is 1 887 bp long including a poly (A) tail was obtained. The AccMRJP3 cDNA encompassed an open-reading frame (ORF) with 1 779 bp encoding 593 amino acids. The un-translated regions (UTR) of the 5' end and 3' end are 46 bp and 160 bp in length,respectively. Similar to AmMRJP3 and AdMRJP3, the putative AccMRJP3 also has a repetitive region. The comparison of the repetitive region of AccMRJP3, AmMRJP3 and AdMRJP3 shows some differences between them. |
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| Keywords: | Apis cerana cerana MRJP3 Gene cloning Sequence analysis |
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