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| 部分湖北海棠种质的鉴定及亲缘关系分析 | |
| 引用本文： | 张靖国,胡红菊,徐育海,罗正荣.部分湖北海棠种质的鉴定及亲缘关系分析[J].华中农业大学学报,2009,28(6). |
| 作者姓名： | 张靖国 胡红菊 徐育海 罗正荣 |
| 作者单位： | 1. 华中农业大学园艺植物生物学教育部重点实验室,武汉,430070;湖北省农业科学院果树茶叶研究所,武汉,430209 2. 湖北省农业科学院果树茶叶研究所,武汉,430209 3. 华中农业大学园艺植物生物学教育部重点实验室,武汉,430070 |
| 基金项目： | 高等学校博士学科点专项科研基金项目 |
| 摘 要： | 用17对SSR引物对来自鄂西地区的兴山、房县、竹溪、宣恩、鹤峰、建始以及山东临沂等地33份湖北海棠种质和2个苹果属近缘种进行了种质鉴定和亲缘关系分析.在33份湖北海棠中共扩增出121个等位基因,平均每个位点7.12个,平均多态性比率为98.3%;除GD15、GD162和Z71981外的14对引物中任意一对均可区分所有供试材料,在17个SSR位点上共检出供试33份湖北海棠试材分属6个基因型,表明湖北海棠种下存在较高的遗传多样性.此外,倍性检测结果表明所有供试材料均为三倍体基因型. |
| 关 键 词： | 湖北海棠 种质鉴定 亲缘关系 |
Germplasm Identification and Genetic Relationship of some Malus hupehensis (Pamp.) Rehd. Accessions |
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| ZHANG Jing-guo,HU Hong-ju,XU Yu-hai,LUO Zheng-rong.Germplasm Identification and Genetic Relationship of some Malus hupehensis (Pamp.) Rehd. Accessions[J].Journal of Huazhong Agricultural University,2009,28(6). | |
| Authors: | ZHANG Jing-guo HU Hong-ju XU Yu-hai LUO Zheng-rong |
| Abstract: | Simple sequence repeat(SSR) markers were used to identify the germplasm and genetic relationship of Malus hupehensis (Pamp.) Rehd. (Tea Crabapple) accessions came from five regions such as Xingshan, Fangxian, Zhuxi, Xuanen, Hefeng and Jianshi, the western of Hubei Province, Linyi in Shandong Province and 2 relative species. A total of 121 putative alleles were amplified from 17 primer-pairs developed and screened from apple on 33 M. hupehensis(Pamp. ) Rehd accessions. All SSR markers were highly polymorphic with a mean of 7. 12 putative alleles per locus and a mean polymorphic percentage of 98. 3. Except GD15. GD162, Z71981, each primer could differentiate in all 33 accessions belonging to 6 genotypes, suggesting high genetic diversity existed in Malus hupehensis (Pamp.) Rehd. Ploidy analysis showed that all accessions were triploid. |
| Keywords: | SSR Malus hupehensis (Pamp ) Rehd germplasm identification genetic relationship SSR |
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